Ethanol improves the transformation efficiency of intact yeast cells
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
1934108
DOI
10.1007/bf00312757
Knihovny.cz E-zdroje
- MeSH
- DNA genetika MeSH
- Escherichia coli genetika MeSH
- ethanol farmakologie MeSH
- plazmidy MeSH
- Saccharomyces cerevisiae genetika růst a vývoj MeSH
- transformace genetická účinky léků MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- DNA MeSH
- ethanol MeSH
A technique is described in which ethanol is used to improve the genetic transformation of intact yeast (Saccharomyces cerevisiae) cells pretreated with LiAc and PEG. Transformation efficiency was increased with increasing concentrations of ethanol with a peak at 10% concentration. The effect varies with different yeast strains and plasmids and up to a maximum of a 15-fold increase was observed.
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