Caesium fluoride-induced changes in the c.d. spectra of synthetic DNA fragments
Language English Country Netherlands Media print
Document type Journal Article
PubMed
2059585
DOI
10.1016/0141-8130(91)90003-d
PII: 0141-8130(91)90003-D
Knihovny.cz E-resources
- MeSH
- Adenine Nucleotides chemistry MeSH
- Cesium chemistry MeSH
- Circular Dichroism MeSH
- DNA chemistry MeSH
- Fluorides * MeSH
- Isomerism MeSH
- Nucleic Acid Conformation MeSH
- Macromolecular Substances MeSH
- Models, Molecular MeSH
- Molecular Sequence Data MeSH
- Oligonucleotides chemistry MeSH
- Polynucleotides chemistry MeSH
- Base Sequence MeSH
- Spectrophotometry, Ultraviolet MeSH
- Thymine Nucleotides chemistry MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Adenine Nucleotides MeSH
- cesium fluoride MeSH Browser
- Cesium MeSH
- DNA MeSH
- Fluorides * MeSH
- Macromolecular Substances MeSH
- Oligonucleotides MeSH
- Polynucleotides MeSH
- Thymine Nucleotides MeSH
Ten DNA fragments containing self-complementary alternating sequences of adenine and thymine differing in length and the starting nucleotide were studied by c.d. spectroscopy. It was found that d(TATATATA) but not d(ATATATAT), d(TATATA), d(CTATATAG) or (dT-dA)20 isomerized into the unusual X-DNA double helix at molar concentrations of CsF in solution. But in contrast to poly(dA-dT), the octamer (dT-dA)4, isomerized very slowly, at relatively low CsF concentrations and the isomerization was strongly dependent on the octamer concentration. A model is proposed to account for the observed properties of the B-to-X isomerization on the oligomer level.
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