Disulfide bridges of bovine spleen cathepsin B
Language English Country Germany Media print
Document type Journal Article
- MeSH
- Cysteine analysis MeSH
- Disulfides analysis MeSH
- Chromatography, Gel MeSH
- Cathepsin B analysis MeSH
- Molecular Sequence Data MeSH
- Cross-Linking Reagents analysis MeSH
- Amino Acid Sequence MeSH
- Cattle MeSH
- Spleen enzymology MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Cysteine MeSH
- Disulfides MeSH
- Cathepsin B MeSH
- Cross-Linking Reagents MeSH
Bovine spleen cathepsin B contains 7 disulfide bridges. Cleavage of the enzyme with cyanogen bromide gives rise to a large and a small fragment. The former contains all disulfide bridges. Their arrangement was determined by analysis of amino-acid sequences and compositions of subfragments prepared by cleavage of the large cyanogen-bromide fragment with trypsin, chymotrypsin and the staphylococcal proteinase using specific methods for the detection of S-S-bonds. Disulfide bridges link together Cys14-Cys43, Cys26-Cys71, Cys62-Cys128, Cys63-Cys67, Cys100-Cys132, Cys108-Cys119 and Cys148-Cys252.
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