The LTR, v-src, LTR provirus generated in the mammalian genome by src mRNA reverse transcription and integration
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
2463374
PubMed Central
PMC247787
DOI
10.1128/jvi.63.2.1015-1018.1989
Knihovny.cz E-zdroje
- MeSH
- experimentální nádory mikrobiologie MeSH
- křečci praví MeSH
- nádorové buňky kultivované analýza MeSH
- onkogenní protein pp60(v-src) MeSH
- onkogeny * MeSH
- proviry genetika izolace a purifikace MeSH
- rekombinace genetická MeSH
- repetitivní sekvence nukleových kyselin MeSH
- Retroviridae - proteiny genetika MeSH
- reverzní transkriptasa metabolismus MeSH
- virové geny * MeSH
- virové proteiny metabolismus MeSH
- viry ptačího sarkomu genetika izolace a purifikace MeSH
- zvířata MeSH
- Check Tag
- křečci praví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- onkogenní protein pp60(v-src) MeSH
- Retroviridae - proteiny MeSH
- reverzní transkriptasa MeSH
- virové proteiny MeSH
Different types of altered proviruses of Rous sarcoma virus (RSV) have been detected in mammalian tumor cell lines. We cloned and sequenced one of these altered proviruses with the structure LTR, v-src, LTR. The presence of an intact viral splice junction, as well as duplications of the chromosomal sequence GCGGGG flanking the two 2-base-pair-deleted LTRs, demonstrated reverse transcription and normal retroviral integration of src mRNA in mammalian cells. In addition, a 1-nucleotide deletion 2 bases upstream from the AAUAAA polyadenylation signal is suspected to be responsible for the absence of a poly(A) track in the src mRNA present in virions of rescued viruses.
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A transformation-competent recombinant between v-src and Rous-associated virus RAV-1
Complete nucleotide sequence of LTR, v-src, LTR provirus H-19
GENBANK
M21954, M21955
