Identification of transformation-specific proteins synthesized in cryptovirogenic mammalian cells
Language English Country Czech Republic Media print
Document type Journal Article
PubMed
2991029
Knihovny.cz E-resources
- MeSH
- Cell Line MeSH
- Sarcoma, Experimental analysis microbiology MeSH
- Rabbits MeSH
- Cricetinae MeSH
- Mesocricetus MeSH
- Chick Embryo MeSH
- Neoplasm Proteins immunology isolation & purification MeSH
- Oncogene Protein pp60(v-src) MeSH
- Protein Kinases isolation & purification MeSH
- Antibodies, Neoplasm immunology MeSH
- Cell Transformation, Viral * MeSH
- Viral Proteins immunology isolation & purification MeSH
- Avian Sarcoma Viruses MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Cricetinae MeSH
- Chick Embryo MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Neoplasm Proteins MeSH
- Oncogene Protein pp60(v-src) MeSH
- Protein Kinases MeSH
- Antibodies, Neoplasm MeSH
- Viral Proteins MeSH
The cryptovirogenic PR-RSH-19 (H-19) hamster cell line, derived originally from a tumour induced by the virus rescued from XC cells by transfection, was studied. The pp60v-src protein was detected by immunoprecipitation with sera from tumour-bearing rabbits (TBR sera) from lysates of H-19 cells. A closely related protein p60 was precipitated by antiserum obtained from hamsters with tumours induced by H-19 cells. The pp60v-src of H-19 cells possesses an associated kinase activity. These results are consistent with the notion that the detected pp60v-src protein is a product of the cryptic sequences in H-19 cells.
Tumor induction by the LTR, v-src, LTR DNA in four B (MHC) congenic lines of chickens
