Structural and functional stability of foreign genes in transgenic tobacco plants
Language English Country Czech Republic Media print
Document type Journal Article
PubMed
2561278
Knihovny.cz E-resources
- MeSH
- DNA genetics MeSH
- Drosophila genetics MeSH
- Escherichia coli genetics MeSH
- Phosphotransferases genetics metabolism MeSH
- Nucleic Acid Hybridization MeSH
- Plants, Toxic MeSH
- Kanamycin MeSH
- Kanamycin Kinase MeSH
- Cloning, Molecular MeSH
- Drug Resistance genetics MeSH
- Plasmids MeSH
- DNA, Recombinant * MeSH
- DNA Restriction Enzymes MeSH
- Rhizobium genetics MeSH
- Plants genetics MeSH
- Nicotiana MeSH
- Transfection * MeSH
- Transformation, Genetic MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- DNA MeSH
- Phosphotransferases MeSH
- Kanamycin MeSH
- Kanamycin Kinase MeSH
- DNA, Recombinant * MeSH
- DNA Restriction Enzymes MeSH
The Drosophila genomic fragment Dm111 and the selectable dominant nptII gene were transferred via a Ti-vector into tobacco plants in order to check the structural and functional stability of transgenes in plants and their progeny. Southern blot analyses clearly showed that transgenes were integrated intact and did not suffer from any gross DNA rearrangements. Contrary to this structural stability, not all of the transgenic plants and their offspring displayed the original and stable expression of the nptII gene. The levels of the NPTII enzyme strongly varied in individual plants and did not depend on the copy number of the nptII gene. Both the non-stable nptII expression during the individual plant development in one original transgenic tobacco and some irregularities in segregation ratios after self-pollination indicated that epigenetic effects due to methylation of DNA modulated the expression of foreign genes in transgenic plants. This conclusion was supported by a spontaneous and 5-azacytidine-stimulated demethylation.
