Structural and functional stability of foreign genes in transgenic tobacco plants
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články
PubMed
2561278
Knihovny.cz E-zdroje
- MeSH
- DNA genetika MeSH
- Drosophila genetika MeSH
- Escherichia coli genetika MeSH
- fosfotransferasy genetika metabolismus MeSH
- hybridizace nukleových kyselin MeSH
- jedovaté rostliny MeSH
- kanamycin MeSH
- kanamycinkinasa MeSH
- klonování DNA MeSH
- léková rezistence genetika MeSH
- plazmidy MeSH
- rekombinantní DNA * MeSH
- restrikční enzymy MeSH
- Rhizobium genetika MeSH
- rostliny genetika MeSH
- tabák MeSH
- transfekce * MeSH
- transformace genetická MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- DNA MeSH
- fosfotransferasy MeSH
- kanamycin MeSH
- kanamycinkinasa MeSH
- rekombinantní DNA * MeSH
- restrikční enzymy MeSH
The Drosophila genomic fragment Dm111 and the selectable dominant nptII gene were transferred via a Ti-vector into tobacco plants in order to check the structural and functional stability of transgenes in plants and their progeny. Southern blot analyses clearly showed that transgenes were integrated intact and did not suffer from any gross DNA rearrangements. Contrary to this structural stability, not all of the transgenic plants and their offspring displayed the original and stable expression of the nptII gene. The levels of the NPTII enzyme strongly varied in individual plants and did not depend on the copy number of the nptII gene. Both the non-stable nptII expression during the individual plant development in one original transgenic tobacco and some irregularities in segregation ratios after self-pollination indicated that epigenetic effects due to methylation of DNA modulated the expression of foreign genes in transgenic plants. This conclusion was supported by a spontaneous and 5-azacytidine-stimulated demethylation.
