Convenience of plasmid R6K higher-copy-number deletion derivative for cloning of larger DNA fragments
Language English Country United States Media print
Document type Journal Article
PubMed
2822553
DOI
10.1007/bf02877215
Knihovny.cz E-resources
- MeSH
- Drug Resistance, Microbial MeSH
- Deoxyribonuclease BamHI MeSH
- Deoxyribonuclease EcoRI MeSH
- Escherichia coli genetics MeSH
- Genetic Vectors * MeSH
- Cloning, Molecular methods MeSH
- Plasmids * MeSH
- DNA, Recombinant * MeSH
- DNA Restriction Enzymes MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Deoxyribonuclease BamHI MeSH
- Deoxyribonuclease EcoRI MeSH
- DNA, Recombinant * MeSH
- DNA Restriction Enzymes MeSH
Vector properties of plasmid pNH602, a higher-copy-number deletion mutant of plasmid R6K, were tested by cloning the 6.5 Mg/mol BamHI pSa fragment carrying determinants of resistance to four antibiotics in the unique BamHI site of pNH602. The resulting in vitro constructed recombinant plasmid pNH606 was found to be stable, conjugative, multicopy (20 copies of pNH606 per E. coli chromosome were estimated) and to ensure the increased expression of different genes responsible for the antibiotic resistance. The pSa fragment inserted in the BamHI site of plasmid pNH602 (located in Tn2660) was proved to be transposable to other replicons. Recombinant plasmid pNH606 was analyzed using restriction enzymes BamHI and EcoRI and its physical and genetic map was constructed.
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Anal Biochem. 1979 Dec;100(2):319-23 PubMed
Plasmid. 1978 Sep;1(4):584-8 PubMed
Folia Microbiol (Praha). 1982;27(2):138-41 PubMed
J Gen Microbiol. 1982 Mar;128(3):529-37 PubMed
Nucleic Acids Res. 1977;4(5):1465-82 PubMed
J Bacteriol. 1970 Oct;104(1):34-44 PubMed
Gene. 1983 Aug;23(2):105-15 PubMed
J Virol. 1974 Nov;14(5):1235-44 PubMed
J Bacteriol. 1976 Sep;127(3):1529-37 PubMed
Plasmid. 1981 Jan;5(1):2-9 PubMed
Folia Microbiol (Praha). 1983;28(5):345-52 PubMed
Plasmid. 1983 Jul;10(1):31-44 PubMed
Microbiol Rev. 1983 Jun;47(2):180-230 PubMed
Virology. 1980 Nov;107(1):89-99 PubMed
Mol Gen Genet. 1982;186(1):10-5 PubMed
Gene. 1982 Nov;20(1):39-49 PubMed
Genetika. 1983 Oct;19(10):1573-81 PubMed
Genetika. 1978 Nov;14(11):1863-75 PubMed
Plasmid. 1979 Jul;2(3):485-92 PubMed
Nucleic Acids Res. 1978 Aug;5(8):2721-8 PubMed
J Bacteriol. 1983 Aug;155(2):722-7 PubMed
J Bacteriol. 1979 Aug;139(2):515-9 PubMed
J Mol Biol. 1968 Feb 14;31(3):519-40 PubMed
Bacteriol Rev. 1968 Mar;32(1):55-83 PubMed
J Bacteriol. 1968 Nov;96(5):1791-5 PubMed
Genetika. 1982;18(8):1263-70 PubMed
J Bacteriol. 1973 May;114(2):577-91 PubMed
Mol Gen Genet. 1982;187(2):187-94 PubMed
Folia Microbiol (Praha). 1986;31(4):257-66 PubMed
Folia Microbiol (Praha). 1985;30(5):407-13 PubMed
Folia Microbiol (Praha). 1977;22(5):353-9 PubMed
