Physiology of a wild strain and high yielding mutants of Streptomyces rimosus, producing oxytetracycline
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu srovnávací studie, časopisecké články
PubMed
2891594
DOI
10.1007/bf02887569
Knihovny.cz E-zdroje
- MeSH
- acetyl-CoA-karboxylasa metabolismus MeSH
- druhová specificita MeSH
- fermentace MeSH
- fosfoenolpyruvátkarboxylasa metabolismus MeSH
- isocitrátdehydrogenasa metabolismus MeSH
- kinetika MeSH
- kyslík analýza MeSH
- mutace * MeSH
- oxid uhličitý analýza MeSH
- oxygenasy metabolismus MeSH
- oxytetracyklin biosyntéza MeSH
- Streptomyces genetika fyziologie MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- acetyl-CoA-karboxylasa MeSH
- anhydrotetracycline oxygenase MeSH Prohlížeč
- fosfoenolpyruvátkarboxylasa MeSH
- isocitrátdehydrogenasa MeSH
- kyslík MeSH
- oxid uhličitý MeSH
- oxygenasy MeSH
- oxytetracyklin MeSH
A wild-type Streptomyces strain, yielding 1 g/L of oxytetracycline was compared with mutants giving up to 7 g/L, using complex media in stirred and shaken culture. Increased production of oxytetracycline was associated with high specific production rates and a longer production period. The superiority of the mutants was associated with changes in morphological behaviour during growth in submerged culture, and in their patterns of growth and respiration, coupled with increased resistance to the product. The productivity of the mutants was sensitive to the rate of stirring, the type of calcium carbonate used in the medium and the type of inoculum. Careful control of these factors was necessary to obtain high yields of oxytetracycline. With the exception of acetyl-CoA carboxylase, the levels of enzymes measured and of amounts of adenylates in the mycelium did not appear to be related to the degree of antibiotic production.
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