On the in vitro neutralization test of Clostridium perfringens type A toxin
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články
PubMed
2900852
Knihovny.cz E-zdroje
- MeSH
- antitoxiny analýza MeSH
- bakteriální toxiny analýza imunologie MeSH
- Clostridium perfringens * enzymologie MeSH
- fosfolipasy typu C antagonisté a inhibitory MeSH
- lidé MeSH
- neutralizační testy * MeSH
- proteiny vázající vápník * MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- alpha toxin, Clostridium perfringens MeSH Prohlížeč
- antitoxiny MeSH
- bakteriální toxiny MeSH
- fosfolipasy typu C MeSH
- proteiny vázající vápník * MeSH
Data are presented on the detection in crude animal and human sera of Cl. perfringens phospholipase C (PLC) inhibitor. When the level of Cl. perfringens type A antitoxin is determined in the in vitro toxin neutralization test the inhibitor is found to decrease PLC activity in the test dose of experimental homologous toxin. The extent of decrease accounts for the variation of results obtained in the in vitro and in vivo toxin neutralization tests. The variation may be cancelled out by introducing a corresponding coefficient to calculate the level of alpha-antitoxin. It is suggested that the isolation and investigation of the PLC inhibitor will contribute to the development of preparations for treatment of gas gangrene due to Cl. perfringens type A.
