With the use of the method Chick Embryotoxicity Screening Test II (CHEST II), the potential neuropeptides L-prolyl-L-leucyl-glycinamide (MIF), cyclo(1-aminocyclo-pentanecarbonyl-L-alanyl)[cyclo(Acp-Ala)] and cyclo(glycyl-L-leucyl)[Cyclo(Gly-Leu)] were tested in the critical developmental periods of d 1.5 to 4 of chick embryogenesis in order to objectively examine their undesirable interactions with the developing morphogenetic systems of the brain, eye, face, body wall, limbs, trunk and heart. All compounds tested showed positive dose- and stage-response relationships. The body wall defects are the prevailing type of malformations.

Department of Pharmacological Propaedeutics and Organic Chemistry Faculty of Pharmacy Charles University Hradec Králové Czechoslovakia

In vitro permeation of micronized and nanonized alaptide from semisolid formulations