The nucleotide sequence of the chicken myb proto-oncogene putative promoter region was determined and compared with the corresponding sequence of the mouse c-myb gene (1). 118 bp upstream from the initiation codon suggested by Gerondakis and Bishop (2) for the chicken c-myb protein, a 124-bp-long conserved element was found (92% identity in chicken and mouse sequences). Sequences homologous to this element were detected on Southern blots of restricted genomic DNAs from mouse, man, lizard, frog, and carp. No hybridization was observed with Drosophila, yeast, or Escherichia coli DNA. In human DNA, sequences homologous to this element were located at the 5' end of the c-myb gene, i.e. in the same position as in the chicken and mouse genes. Several lines of evidence suggest that the element is not a coding exon of a gene overlapping the c-myb gene. It may be of importance that one of the DNase I-sensitive sites and several c-myb mRNA cap sites localized recently in the mouse c-myb gene (3,4) lie within this region. It is suggested that this evolutionarily conserved element is involved in the regulation of myb proto-oncogene expression in vertebrates.

Institute of Molecular Genetics Czechoslovak Academy of Sciences Prague

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Anal Biochem. 1985 May 15;147(1):114-9 PubMed

Cell. 1985 Jun;41(2):449-56 PubMed

EMBO J. 1985 Aug;4(8):2003-8 PubMed

Nature. 1986 Feb 13-19;319(6054):604-6 PubMed

Nucleic Acids Res. 1986 Feb 11;14(3):1319-24 PubMed

Proc Natl Acad Sci U S A. 1986 May;83(10):3204-8 PubMed

Proc Natl Acad Sci U S A. 1986 Jul;83(13):4685-9 PubMed

Science. 1986 Jul 18;233(4761):347-51 PubMed

Nucleic Acids Res. 1986 Jul 11;14(13):5309-20 PubMed

EMBO J. 1986 Aug;5(8):1903-11 PubMed

Mol Cell Biol. 1986 Jan;6(1):62-9 PubMed

Mol Cell Biol. 1986 Nov;6(11):3677-84 PubMed

Proc Natl Acad Sci U S A. 1986 Dec;83(24):9636-40 PubMed

Folia Biol (Praha). 1987;33(1):1-10 PubMed

EMBO J. 1987 Jun;6(6):1643-51 PubMed

Science. 1987 Sep 18;237(4821):1473-6 PubMed

Gene. 1987;54(2-3):155-65 PubMed

J Mol Biol. 1987 Jul 20;196(2):261-82 PubMed

EMBO J. 1987 Oct;6(10):3085-90 PubMed

Nature. 1987 Dec 24-31;330(6150):701-2 PubMed

J Mol Evol. 1987;26(1-2):148-56 PubMed

Oncogene. 1988 Mar;2(3):267-72 PubMed

FEBS Lett. 1988 May 9;232(1):227-34 PubMed

Oncogene. 1987;1(4):395-401 PubMed

Oncogene. 1988 Jun;2(6):545-51 PubMed

Mol Cell Biol. 1982 Jun;2(6):617-24 PubMed

Methods Cell Biol. 1975;12:39-44 PubMed

Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PubMed

Nature. 1979 Oct 11;281(5731):452-5 PubMed

Proc Natl Acad Sci U S A. 1980 Sep;77(9):5177-81 PubMed

J Virol. 1980 Oct;36(1):162-70 PubMed

J Virol. 1981 Nov;40(2):450-5 PubMed

Nucleic Acids Res. 1982 Jan 22;10(2):459-72 PubMed

J Virol. 1982 Jan;41(1):250-7 PubMed

Proc Natl Acad Sci U S A. 1982 Apr;79(7):2194-8 PubMed

Cell. 1982 Dec;31(2 Pt 1):453-63 PubMed

Nucleic Acids Res. 1983 Jan 25;11(2):349-68 PubMed

Proc Natl Acad Sci U S A. 1983 Feb;80(3):726-30 PubMed

J Mol Biol. 1982 Dec 25;162(4):729-73 PubMed

EMBO J. 1983;2(7):1073-8 PubMed

Exp Cell Res. 1983 Nov;149(1):151-62 PubMed

Cancer Res. 1984 Feb;44(2):442-6 PubMed

Gene. 1983 Dec;26(1):101-6 PubMed

Cell. 1984 Jun;37(2):537-47 PubMed

Nature. 1984 Jul 19-25;310(5974):249-51 PubMed

Mol Cell Biol. 1984 Jul;4(7):1206-12 PubMed

Nucleic Acids Res. 1985 Oct 25;13(20):7207-21 PubMed

Transcription of the chicken myb proto-oncogene starts within a CpG island

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