Structural studies of mannans from the cell walls of the pathogenic yeasts Candida albicans serotypes A and B and Candida parapsilosis
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu srovnávací studie, časopisecké články
PubMed
3285998
DOI
10.1016/s0008-6215(00)90858-9
PII: S0008-6215(00)90858-9
Knihovny.cz E-zdroje
- MeSH
- buněčná stěna analýza MeSH
- Candida albicans analýza patogenita MeSH
- Candida analýza patogenita MeSH
- druhová specificita MeSH
- konformace sacharidů MeSH
- magnetická rezonanční spektroskopie MeSH
- mannany izolace a purifikace MeSH
- metylace MeSH
- molekulární sekvence - údaje MeSH
- sacharidové sekvence MeSH
- sérotypizace MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- mannany MeSH
A comparative study of three cell-wall mannans, of Candida albicans serotypes A and B and Candida parapsilosis, by means of methylation analysis supports a model of yeast mannans as having an alpha-(1----6)-linked backbone with some units (depending on the origin of the mannan) being substituted at O-2 with oligosaccharides joined by alpha-(1---2) and, to a lesser extent, by alpha-(1----3) glycosidic bonds. Branching points in the side chains of Candida albicans mannans were found in substantial proportions for the first time, and the corresponding branched hexasaccharides were isolated by means of acetolysis and subsequent gel filtration. 13C-N.m.r. spectroscopy of the mannans, as well as a 1H-n.m.r. spectroscopic study of the oligosaccharides obtained on acetolysis of the mannans, led to results that agreed with those of methylation analysis.
Citace poskytuje Crossref.org
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