Production of toxic antigens in dialyzed cultures of microorganisms
Language English Country United States Media print
Document type Journal Article, Review
PubMed
3286420
DOI
10.1007/bf02928083
Knihovny.cz E-resources
- MeSH
- Antigens, Bacterial biosynthesis MeSH
- Botulinum Toxins biosynthesis MeSH
- Clostridium botulinum growth & development MeSH
- Clostridium tetani growth & development MeSH
- Dialysis instrumentation methods MeSH
- Culture Media MeSH
- Tetanus Toxin biosynthesis MeSH
- Publication type
- Journal Article MeSH
- Review MeSH
- Names of Substances
- Antigens, Bacterial MeSH
- Botulinum Toxins MeSH
- Culture Media MeSH
- Tetanus Toxin MeSH
Data on the production of clostridial toxins in dialyzed cultures are summarized. Principal modifications of this cultivation technique suitable for both research and production are shown. If toxins are released from the cells by autolysis (neurotoxins of Clostridium tetani, C. botulinum; lethal factors of C. novyi and C. sordellii), a 10-fold increase of the antigen concentration in filtrates of dialyzed cultures is found in comparison with normal cultures. If toxins are excreted already during growth (lethal factors of C. perfringens type A, C. septicum), the positive effect of the technique is less significant. A dialyzed culture ensures a well-balanced production of toxic filtrates that contain highly concentrated, relatively pure and strongly immunogenic antigens.
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J Immunol. 1953 Jan;70(1):1-5 PubMed
J Bacteriol. 1957 Dec;74(6):718-27 PubMed
Ann Inst Pasteur (Paris). 1954 Dec;87(6):599-616 PubMed
Appl Microbiol. 1963 Nov;11(6):506-12 PubMed
Biochem J. 1948;42(4):633-40 PubMed
J Hyg Epidemiol Microbiol Immunol. 1978;22(1):63-72 PubMed
Can J Med Sci. 1952 Apr;30(2):155-6 PubMed
J Immunol. 1950 Aug;65(2):175-83 PubMed
Appl Microbiol. 1972 Jul;24(1):108-13 PubMed
Science. 1947 May 30;105(2735):582-3 PubMed
Bacteriol Rev. 1969 Mar;33(1):1-47 PubMed
J Bacteriol. 1951 Apr;61(4):507-13 PubMed
Nature. 1946 Aug 17;158:238 PubMed
J Gen Microbiol. 1958 Jun;18(3):747-50 PubMed
Med Dosw Mikrobiol. 1964;16:323-30 PubMed
Boll Ist Sieroter Milan. 1958 Sep-Oct;37(9-10):401-13 PubMed
Can J Microbiol. 1954 Oct;1(2):108-17 PubMed
Appl Microbiol. 1969 Apr;17(4):639-40 PubMed
J Bacteriol. 1960 Mar;79:372-8 PubMed
J Comp Pathol. 1951 Apr;61(2):150-4 PubMed
C R Hebd Seances Acad Sci. 1956 Jan 23;242(4):574-6 PubMed
C R Seances Soc Biol Fil. 1952 Aug;146(15-16):1298-1301 PubMed
Zh Mikrobiol Epidemiol Immunobiol. 1961 Feb;32:16-60 PubMed
Zh Mikrobiol Epidemiol Immunobiol. 1957 Mar;28(3):67-70 PubMed
Science. 1949 Sep 9;110(2854):259 PubMed
Nature. 1950 Oct 28;166(4226):739-40 PubMed
Folia Microbiol (Praha). 1986;31(5):382-6 PubMed
J Bacteriol. 1967 Nov;94(5):1327-33 PubMed
Schweiz Z Pathol Bakteriol. 1959;22(2):224-8 PubMed
Science. 1951 Nov 23;114(2969):549-50 PubMed
J Immunol. 1956 Jun;76(6):393-8 PubMed
