Automated 3'-H-phosphonate synthesis of oligodeoxyribonucleotides using amidine protected purine synthons
Language English Country Great Britain, England Media print
Document type Journal Article
PubMed
3697126
Knihovny.cz E-resources
- MeSH
- Amidines * MeSH
- Indicators and Reagents MeSH
- Oligodeoxyribonucleotides chemical synthesis MeSH
- Organophosphonates * MeSH
- Purines * MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Amidines * MeSH
- Indicators and Reagents MeSH
- Oligodeoxyribonucleotides MeSH
- Organophosphonates * MeSH
- Purines * MeSH
Side reaction during phosphitylation of 5'-O-dimethoxytrityl-N2-isobutyryl-2'-deoxyguanosine was observed. As a suitable dG synthon, 5'-O-dimethoxytrityl-N2-dimethylaminomethyl-ene-3'-H-phosphonate was developed and used, along with 3'-H-phosphonates of 5'-O-dimethoxytrityl derivatives of dT, N4--benzoyl-dC and N6-dimethylaminoethylidene-dA, in automated synthesis of several oligodeoxyribonucleotides containing 30-40 bases.
Transcription of the chicken myb proto-oncogene starts within a CpG island
