Automated 3'-H-phosphonate synthesis of oligodeoxyribonucleotides using amidine protected purine synthons
Jazyk angličtina Země Velká Británie, Anglie Médium print
Typ dokumentu časopisecké články
PubMed
3697126
Knihovny.cz E-zdroje
- MeSH
- amidiny * MeSH
- indikátory a reagencie MeSH
- oligodeoxyribonukleotidy chemická syntéza MeSH
- organofosfonáty * MeSH
- puriny * MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- amidiny * MeSH
- indikátory a reagencie MeSH
- oligodeoxyribonukleotidy MeSH
- organofosfonáty * MeSH
- puriny * MeSH
Side reaction during phosphitylation of 5'-O-dimethoxytrityl-N2-isobutyryl-2'-deoxyguanosine was observed. As a suitable dG synthon, 5'-O-dimethoxytrityl-N2-dimethylaminomethyl-ene-3'-H-phosphonate was developed and used, along with 3'-H-phosphonates of 5'-O-dimethoxytrityl derivatives of dT, N4--benzoyl-dC and N6-dimethylaminoethylidene-dA, in automated synthesis of several oligodeoxyribonucleotides containing 30-40 bases.
Transcription of the chicken myb proto-oncogene starts within a CpG island
