Replication of transformation-defective mutants of the Prague strain of Rous sarcoma virus and isolation of a td mutant from duck-adapted PR-RSV-C
Language English Country Czech Republic Media print
Document type Journal Article
PubMed
6153995
Knihovny.cz E-resources
- MeSH
- Ducks MeSH
- Cells, Cultured MeSH
- Mutation * MeSH
- Virus Replication * MeSH
- RNA-Directed DNA Polymerase analysis MeSH
- Cell Transformation, Viral * MeSH
- Avian Sarcoma Viruses genetics isolation & purification MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- RNA-Directed DNA Polymerase MeSH
The replication of transformation-defective mutants of the Prague strain of Rous sarcoma virus subgroup C was studied using roller cultures. Under such conditions, 10(5)--10(6) infectous units of virus per 0.2 ml were produced, as revealed in both the reverse transcriptase and 16Q complementation tests. A new td daPR-RSV-C mutant was isolated from duck-adapted PR-RSV-C. This mutant replicated in roller cultures with equal efficiency as the original td PR-RSV-C. It was verified that td daPR-RSV-C does not transform chicken fibroblasts, is not oncogenic for 3-week-old chickens and has subgroup C host-range specificity. Both td mutants replicate in duck cells and reach the same titres.
Tumor induction by the LTR, v-src, LTR DNA in four B (MHC) congenic lines of chickens
