The histochemical pattern of mechanically or chemically injured rabbit cornea after aprotinin treatment: relationships with the plasmin concentration of the tear fluid
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články
PubMed
7689549
DOI
10.1007/bf00157808
Knihovny.cz E-zdroje
- MeSH
- aprotinin farmakologie terapeutické užití MeSH
- beta-galaktosidasa metabolismus MeSH
- dipeptidylpeptidasy a tripeptidylpeptidasy metabolismus MeSH
- hydrolasy metabolismus MeSH
- králíci MeSH
- lyzozomy enzymologie MeSH
- neutrofily enzymologie MeSH
- plasmin analýza MeSH
- poranění rohovky * MeSH
- slzy enzymologie MeSH
- vřed rohovky chemicky indukované farmakoterapie enzymologie MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- aprotinin MeSH
- beta-galaktosidasa MeSH
- dipeptidylpeptidasy a tripeptidylpeptidasy MeSH
- hydrolasy MeSH
- plasmin MeSH
Plasmin, a serine protease, was recently found to be involved in corneal ulcerative processes in humans and rabbits. In our experiments, plasmin activity was found in the tear fluid after mechanical and chemical damage of the rabbit cornea, such as de-epithelization and burning with alkali. The plasmin concentrations in the tear fluid were dependent on the severity of injury. The highest plasmin activity (2.0-3.0 micrograms ml-1) occurred after severe alkali damage to large areas of the cornea, and the lowest activity (0.4-1.0 micrograms ml-1) after mechanical injury (de-epithelization). Plasmin concentrations up to 1.0 micrograms ml-1 were associated with increased activities of lysosomal hydrolases in epithelial cells and keratocytes beneath the epithelium. Plasmin activities increased as the inflammatory reaction developed. When plasmin activity in the tear fluid was higher than 1.0 micrograms ml-1, inflammatory cells were found in the corneal stroma. Levels of 1.5-2.0 micrograms ml-1 were connected with higher numbers of inflammatory cells (particularly polymorphonuclear leukocytes) with increased activities of lysosomal hydrolases. Very high plasmin activities (2.5-3.0 micrograms ml-1) accompanied corneal ulcerative processes. The local application of aprotinin (Trasylol, Bayer), an inhibitor of plasmin, and also of some other proteases, was found to be necessary for the healing of severe corneal injuries in which highly elevated plasmin activity in the tear fluid and inflammatory cellulization of the cornea occurred (severe damage).(ABSTRACT TRUNCATED AT 250 WORDS)
Zobrazit více v PubMed
Histochemistry. 1992;97(1):69-76 PubMed
Acta Ophthalmol (Copenh). 1985 Jun;63(3):346-50 PubMed
J Pathol Bacteriol. 1955 Jan-Apr;69(1-2):283-93 PubMed
Histochemistry. 1986;84(1):67-71 PubMed
Arzneimittelforschung. 1983;33(4):479-94 PubMed
J Histochem Cytochem. 1981 Mar;29(3A Suppl):481-93 PubMed
Arch Ophthalmol. 1990 Dec;108(12):1758-63 PubMed
Acta Ophthalmol (Copenh). 1987 Feb;65(1):3-12 PubMed
Invest Ophthalmol Vis Sci. 1980 Oct;19(10):1204-21 PubMed
Invest Ophthalmol Vis Sci. 1991 Jun;32(7):2073-8 PubMed
Histochem J. 1985 Oct;17(10):1063-89 PubMed
Graefes Arch Clin Exp Ophthalmol. 1989;227(2):184-7 PubMed
Histochemistry. 1989;92(5):441-8 PubMed
Invest Ophthalmol Vis Sci. 1985 Apr;26(4):511-24 PubMed
Anal Biochem. 1981 Mar 1;111(2):276-82 PubMed
Acta Ophthalmol (Copenh). 1990 Oct;68(5):508-14 PubMed
Invest Ophthalmol Vis Sci. 1983 Oct;24(10):1358-66 PubMed
Acta Histochem Suppl. 1984;30:9-28 PubMed
Curr Eye Res. 1991 May;10(5):381-98 PubMed
Exp Eye Res. 1990 Jul;51(1):1-9 PubMed
