DNA fingerprint analysis of drug resistant Mycobacterium tuberculosis strains isolated in the Czech Republic
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
7812250
Knihovny.cz E-resources
- MeSH
- Drug Resistance, Microbial genetics MeSH
- Antitubercular Agents pharmacology MeSH
- DNA, Bacterial genetics MeSH
- DNA Fingerprinting * MeSH
- Humans MeSH
- Tuberculosis, Multidrug-Resistant microbiology MeSH
- Mycobacterium tuberculosis classification drug effects genetics MeSH
- Polymorphism, Restriction Fragment Length * MeSH
- Bacterial Typing Techniques MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czechoslovakia MeSH
- Names of Substances
- Antitubercular Agents MeSH
- DNA, Bacterial MeSH
DNA fingerprints were established in 12 drug resistant and 12 susceptible M. tuberculosis strains collected from patients residing in Prague, Czech Republic. The Restriction Fragment Length Polymorphism (RFLP) technique was based on the detection of the insertion sequence IS6110 in PvuII digested chromosomal DNA. All investigated strains possessed at least 5 copies of IS6110 ranging from 5 to 15 copies in the drug resistant isolates and 5 to 11 copies in susceptible isolates. Three multidrug resistant strains displayed identical fingerprints (5 bands), and two strains resistant to pyrazinamid had the same banding pattern (12 bands). The remaining isolates differed either in number or location of the IS6110 copies. Thorough epidemiological analysis did not furnish proof of direct contact among these patients.
