Photodynamic sensitizers assay: rapid and sensitive iodometric measurement
Language English Country Switzerland Media print
Document type Journal Article
PubMed
7875246
DOI
10.1007/bf01929349
Knihovny.cz E-resources
- MeSH
- Photosensitizing Agents analysis MeSH
- Iodides analysis chemistry MeSH
- Kinetics MeSH
- Oxygen analysis chemistry MeSH
- Humans MeSH
- Molybdenum MeSH
- Hydrogen Peroxide chemistry MeSH
- Singlet Oxygen MeSH
- Spectrophotometry methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- ammonium molybdate MeSH Browser
- Photosensitizing Agents MeSH
- Iodides MeSH
- Oxygen MeSH
- Molybdenum MeSH
- Hydrogen Peroxide MeSH
- Singlet Oxygen MeSH
A rapid, sensitive and simple spectrophotometric method for the detection of 1O2 produced by photodynamic photosensitizers in slightly acid and air-saturated aqueous solutions has been developed. The method is based on the reaction of 1O2 (produced by photodynamic processes) with I- in the presence of ammonium molybdate as a catalyst. The reaction product I3-, proportional to 1O2, is followed spectrophotometrically at 355 nm. Several ways of avoiding interference with other oxidizing compounds, either present before or produced during the irradiation, are described.
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