Isolation of group A human rotaviruses from faecal specimens in monkey MA-104 cells
Language English Country Switzerland Media print
Document type Journal Article
PubMed
8067315
Knihovny.cz E-resources
- MeSH
- Cell Line MeSH
- Cytopathogenic Effect, Viral MeSH
- Feces microbiology MeSH
- Gastroenteritis microbiology MeSH
- Haplorhini MeSH
- Virus Cultivation MeSH
- Humans MeSH
- RNA, Viral analysis MeSH
- Rotavirus Infections diagnosis microbiology MeSH
- Rotavirus growth & development isolation & purification MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Czech Republic MeSH
- Slovakia MeSH
- Names of Substances
- RNA, Viral MeSH
Human rotaviruses present in faecal specimens of patients with gastroenteritis were isolated and adapted to growth in MA-104 cells in roller tubes using trypsin pretreatment. Storage temperature-dependent differences were found in the extent of cytopathic effect. Multiplication of viruses in cell cultures was confirmed by immunoelectron microscopy and ELISA. Electrophoresis of rotavirus RNA in polyacrylamide gel was used to verify the identity of the electropherotypes determined in original faecal specimens and in final cell passages.
