Expression of the gene for tumor necrosis factor-beta but not for tumor necrosis factor-alpha is impaired in tumor-bearing mice
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
8242763
DOI
10.1006/cimm.1993.1283
PII: S0008874983712839
Knihovny.cz E-resources
- MeSH
- Sarcoma, Experimental genetics metabolism MeSH
- Gene Expression MeSH
- Lymphotoxin-alpha genetics MeSH
- RNA, Messenger biosynthesis MeSH
- Mice, Inbred C57BL MeSH
- Mice MeSH
- Macrophages, Peritoneal metabolism MeSH
- Spleen metabolism MeSH
- T-Lymphocytes metabolism MeSH
- Tumor Necrosis Factor-alpha genetics MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Lymphotoxin-alpha MeSH
- RNA, Messenger MeSH
- Tumor Necrosis Factor-alpha MeSH
Spleen cells from mice bearing progressively growing syngeneic sarcomas are immunologically hyporeactive and respond by significantly decreased proliferative response to stimulation with mitogens and cytokines. Here we show that these hyporeactive cells synthesize, after mitogen stimulation, comparable amount of mRNA for tumor necrosis factor (TNF)-alpha as do cells from control mice. However, stimulated spleen cells from the same tumor-bearing mice produce considerably less mRNA for TNF-beta than cells from control mice. These observations were further confirmed using purified peritoneal macrophages and enriched splenic T cells. The results thus demonstrate a distinct regulation of expression of genes for TNF-alpha and TNF-beta, two functionally very similar cytokines, and simultaneously a selective impairment of T-cell function in the course of growth of syngeneic tumors in mice.
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