Some leukocyte effector cell-surface molecules movement toward the adjoining target cells takes place during the reaction of NK cytotoxicity (NK R). The majority of the moving molecules are usually anchored via a divalent-ion-dependent interaction (PMM-M2+). The released PMM-M2+ can interact also with the secreted tumor necrosis factor alfa (TNF-alpha). In agreement with PMM-M2+ movement, the number of TNF-alpha binding sites on the target cell surface increases during NK R. In addition, antibodies against PMM-M2+, as well as D-mannose- or N-acetyl-D-glucosamine-terminated oligosaccharides of PMM-M2+ inhibit NK R. A more detailed analysis of PMM-M2+ with monoclonal antibodies used flow cytometry and cell-surface biotinylation. Only 3 of 31 tested CD antigens (CD2, LAK-1 and CD45) were passed through this first strongly restricted experimental screening. The EDTA-released LAK-1 antigen, but not CD2 and CD45, interact with TNF-alpha and cell surface via a mannose-inhibitable interaction dependent on the presence of Ca2+ ions. The mechanism of possible participation of PMM-M2+ in cytotoxic events is discussed in relation to Ca2+ influx and subsequent cytolysin secretion.

Department of Immunology Institute of Microbiology Academy of Sciences of the Czech Republic Prague

Zobrazit více v PubMed

Nature. 1981 Jan 15;289(5794):181-4 PubMed

Immunol Today. 1991 Oct;12(10):343-5 PubMed

J Immunol. 1982 Oct;129(4):1782-7 PubMed

Proc Natl Acad Sci U S A. 1983 Sep;80(18):5762-6 PubMed

Gan. 1983 Aug;74(4):554-67 PubMed

J Immunol. 1990 Feb 1;144(3):1004-9 PubMed

J Biol Chem. 1974 Sep 10;249(17):5536-43 PubMed

Eur J Immunol. 1991 Jan;21(1):231-4 PubMed

Immunogenetics. 1981 Mar 1;12(5-6):587-99 PubMed

J Cell Biol. 1989 Apr;108(4):1407-18 PubMed

Mol Immunol. 1986 Nov;23(11):1211-4 PubMed

Proc Natl Acad Sci U S A. 1990 Jul;87(13):5021-5 PubMed

J Exp Med. 1986 Oct 1;164(4):1193-205 PubMed

Int J Cancer. 1989 Mar 15;43(3):501-7 PubMed

J Immunol. 1981 Apr;126(4):1516-21 PubMed

J Immunol. 1987 Mar 1;138(5):1494-501 PubMed

Biochem Biophys Res Commun. 1989 Oct 16;164(1):271-6 PubMed

Immunol Rev. 1981;56:51-88 PubMed

J Immunol. 1990 Feb 15;144(4):1538-43 PubMed

J Biol Chem. 1976 Sep 10;251(17):5292-9 PubMed

J Clin Invest. 1990 Aug;86(2):416-23 PubMed

J Biol Chem. 1980 Jul 25;255(14):6820-5 PubMed

Biochim Biophys Acta. 1985 Oct 30;847(1):108-14 PubMed

Cold Spring Harb Symp Quant Biol. 1976;40:49-63 PubMed

Cancer Res. 1990 Oct 1;50(19):6285-90 PubMed

J Exp Med. 1987 Aug 1;166(2):319-26 PubMed

Biochemistry. 1991 Feb 12;30(6):1561-71 PubMed

Cesk Neurol Neurochir. 1991 Sep;54(4):220-2 PubMed

Nature. 1986 Sep 18-24;323(6085):262-4 PubMed

J Immunol. 1982 Jan;128(1):289-95 PubMed

Mol Immunol. 1983 Dec;20(12):1267-72 PubMed

Eur J Biochem. 1993 May 1;213(3):1303-13 PubMed

Biochemistry. 1984 Feb 28;23(5):815-22 PubMed

J Supramol Struct. 1977;7(3-4):339-51 PubMed

J Membr Biol. 1989 Mar;107(3):277-86 PubMed

J Immunol. 1982 Oct;129(4):1384-90 PubMed

FEBS Lett. 1987 Jun 22;218(1):167-72 PubMed

J Immunol. 1985 Jul;135(1):659-64 PubMed

Eur J Immunol. 1991 Apr;21(4):1077-80 PubMed

Cell Immunol. 1987 May;106(2):223-33 PubMed

Biochem J. 1990 Sep 15;270(3):755-60 PubMed

J Immunol. 1990 Dec 1;145(11):3578-88 PubMed

Association of the leukemia inhibitory factor gene mutation and the antiphospholipid antibodies in the peripheral blood of infertile women

Role of the leukemia-inhibitory factor gene mutations in infertile women: the embryo-endometrial cytokine cross talk during implantation--a delicate homeostatic equilibrium