Nonisotopic ultrastructural mapping of transcription sites within the nucleolus
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
8359221
DOI
10.1006/excr.1993.1247
PII: S0014-4827(83)71247-4
Knihovny.cz E-zdroje
- MeSH
- buněčné jadérko metabolismus ultrastruktura MeSH
- elektronová mikroskopie MeSH
- genetická transkripce * MeSH
- HeLa buňky MeSH
- lidé MeSH
- RNA ribozomální biosyntéza MeSH
- techniky in vitro MeSH
- uridintrifosfát analogy a deriváty metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 5-bromouridine triphosphate MeSH Prohlížeč
- RNA ribozomální MeSH
- uridintrifosfát MeSH
A nonradioactive ultrastructural method based on the incorporation of 5-bromouridine-5'-triphosphate into the RNA of streptolysin O-permeabilized cultured HeLa cells is described and used for the visualization of rRNA transcription sites. Even though the method provides much better resolution than ultrastructural autoradiography, the results obtained do not allow the assignment of rRNA transcription to a single nucleolar structural component. We locate the rRNA transcription sites at the border region of fibrillar centers with dense fibrillar components. In addition, the method represents a convenient tool for the in situ immunodetection of extranucleolar RNA synthesis.
Citace poskytuje Crossref.org
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