Nonisotopic ultrastructural mapping of transcription sites within the nucleolus
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
8359221
DOI
10.1006/excr.1993.1247
PII: S0014-4827(83)71247-4
Knihovny.cz E-resources
- MeSH
- Cell Nucleolus metabolism ultrastructure MeSH
- Microscopy, Electron MeSH
- Transcription, Genetic * MeSH
- HeLa Cells MeSH
- Humans MeSH
- RNA, Ribosomal biosynthesis MeSH
- In Vitro Techniques MeSH
- Uridine Triphosphate analogs & derivatives metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 5-bromouridine triphosphate MeSH Browser
- RNA, Ribosomal MeSH
- Uridine Triphosphate MeSH
A nonradioactive ultrastructural method based on the incorporation of 5-bromouridine-5'-triphosphate into the RNA of streptolysin O-permeabilized cultured HeLa cells is described and used for the visualization of rRNA transcription sites. Even though the method provides much better resolution than ultrastructural autoradiography, the results obtained do not allow the assignment of rRNA transcription to a single nucleolar structural component. We locate the rRNA transcription sites at the border region of fibrillar centers with dense fibrillar components. In addition, the method represents a convenient tool for the in situ immunodetection of extranucleolar RNA synthesis.
References provided by Crossref.org
Visualization of the Nucleolus Using Ethynyl Uridine
Fluctuations of pol I and fibrillarin contents of the nucleoli
Reproduction of the FC/DFC units in nucleoli
SUV39h-independent association of HP1 beta with fibrillarin-positive nucleolar regions
