Modified simplified method for isolation of lysostaphin from the culture filtrate of Staphylococcus staphylolyticus
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
8365700
DOI
10.1007/bf02814386
Knihovny.cz E-zdroje
- MeSH
- chromatografie iontoměničová MeSH
- filtrace MeSH
- gelová chromatografie MeSH
- kultivační média MeSH
- lysostafin izolace a purifikace MeSH
- mikrobiologické techniky MeSH
- molekulární sekvence - údaje MeSH
- sacharidové sekvence MeSH
- sekvence aminokyselin MeSH
- Staphylococcus chemie růst a vývoj MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- kultivační média MeSH
- lysostafin MeSH
The present paper reports a modified method for isolation of lysostaphin--a bacteriolytic agent with specific affinity for staphylococcal cell wall. The proposed purification scheme includes three steps. The first procedure is ultrafiltration through a membrane filter giving a yield of 75.6%. The result of ultrafiltration is a concentrated, 10-times purified preparation of lysostaphin with specific activity 0.62 U/mg which can be used for digestion of S. aureus cells. Further step, performed by ion-exchange chromatography on DEAE-cellulose, yields a 60-times purified preparation containing a mixture of enzyme components of lysostaphin. The yield of this step is 47.2%, the preparation contains 3.54 U/mg protein. Using gel filtration on Sephadex G-50 a component with hexosaminidase activity was separated from the endopeptidase component on the basis of molar mass difference. A 270-times purified preparation of lysostaphin-endopeptidase with minimum of contaminating substances was obtained in this step. The yield of gel filtration was 22.1%, specific activity increased up to 16.3 U/mg protein.
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