Unidirectional arginine transport in reconstituted plasma-membrane vesicles from yeast overexpressing CAN1
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- arginin metabolismus MeSH
- biologický transport MeSH
- buněčná membrána metabolismus MeSH
- exprese genu * MeSH
- kinetika MeSH
- liposomy metabolismus MeSH
- membránové transportní proteiny genetika metabolismus MeSH
- plazmidy MeSH
- Saccharomyces cerevisiae - proteiny MeSH
- Saccharomyces cerevisiae genetika metabolismus MeSH
- transformace genetická MeSH
- transportní systémy aminokyselin * MeSH
- transportní systémy pro bazické aminokyseliny MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- arginin MeSH
- arginine permease MeSH Prohlížeč
- CAN1 protein, S cerevisiae MeSH Prohlížeč
- liposomy MeSH
- membránové transportní proteiny MeSH
- Saccharomyces cerevisiae - proteiny MeSH
- transportní systémy aminokyselin * MeSH
- transportní systémy pro bazické aminokyseliny MeSH
Amino acids are accumulated in Saccharomyces cerevisiae by strictly unidirectional influx systems. To see whether cellular compartmentation causes this unusual amino-acid-transport behaviour, arginine transport was studied in plasma-membrane vesicles. The arginine permease gene CAN1 was overexpressed in S. cerevisiae RH218a and in a permease-deficient mutant RS453 (can1). Reconstituted plasma-membrane vesicles from these transformants, energized by incorporated cytochrome-c oxidase, showed 3-4-fold increased rates of arginine uptake compared to vesicles from wild-type cells. The KT values were 32.5 microM in vesicles from wild-type and 28.6 microM in vesicles from transformed cells; the corresponding in vivo values were 17.5 microM and 11.4 microM, respectively. It could be demonstrated that unidirectional arginine transport and accumulation also exist in vesicles; thus, unidirectional influx is not related to cellular compartmentation.
Citace poskytuje Crossref.org
General and molecular microbiology and microbial genetics in the IM CAS
