Unidirectional arginine transport in reconstituted plasma-membrane vesicles from yeast overexpressing CAN1
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Arginine metabolism MeSH
- Biological Transport MeSH
- Cell Membrane metabolism MeSH
- Gene Expression * MeSH
- Kinetics MeSH
- Liposomes metabolism MeSH
- Membrane Transport Proteins genetics metabolism MeSH
- Plasmids MeSH
- Saccharomyces cerevisiae Proteins MeSH
- Saccharomyces cerevisiae genetics metabolism MeSH
- Transformation, Genetic MeSH
- Amino Acid Transport Systems * MeSH
- Amino Acid Transport Systems, Basic MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Arginine MeSH
- arginine permease MeSH Browser
- CAN1 protein, S cerevisiae MeSH Browser
- Liposomes MeSH
- Membrane Transport Proteins MeSH
- Saccharomyces cerevisiae Proteins MeSH
- Amino Acid Transport Systems * MeSH
- Amino Acid Transport Systems, Basic MeSH
Amino acids are accumulated in Saccharomyces cerevisiae by strictly unidirectional influx systems. To see whether cellular compartmentation causes this unusual amino-acid-transport behaviour, arginine transport was studied in plasma-membrane vesicles. The arginine permease gene CAN1 was overexpressed in S. cerevisiae RH218a and in a permease-deficient mutant RS453 (can1). Reconstituted plasma-membrane vesicles from these transformants, energized by incorporated cytochrome-c oxidase, showed 3-4-fold increased rates of arginine uptake compared to vesicles from wild-type cells. The KT values were 32.5 microM in vesicles from wild-type and 28.6 microM in vesicles from transformed cells; the corresponding in vivo values were 17.5 microM and 11.4 microM, respectively. It could be demonstrated that unidirectional arginine transport and accumulation also exist in vesicles; thus, unidirectional influx is not related to cellular compartmentation.
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General and molecular microbiology and microbial genetics in the IM CAS
