Cell calcium handling and intracellular pH regulation in hereditary hypertriglyceridemic rats: reduced platelet response to thrombin stimulation
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
8761314
DOI
10.1016/0024-3205(96)00371-2
PII: 0024320596003712
Knihovny.cz E-zdroje
- MeSH
- buněčná membrána metabolismus MeSH
- hypertenze metabolismus MeSH
- hypertriglyceridemie genetika metabolismus MeSH
- inbrední kmeny potkanů MeSH
- iontové kanály metabolismus MeSH
- iontový transport MeSH
- koncentrace vodíkových iontů MeSH
- krysa rodu Rattus MeSH
- mangan metabolismus MeSH
- Na(+)-H(+) antiport metabolismus MeSH
- potkani Wistar MeSH
- trombin farmakologie MeSH
- trombocyty cytologie účinky léků MeSH
- vápník metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- iontové kanály MeSH
- mangan MeSH
- Na(+)-H(+) antiport MeSH
- trombin MeSH
- vápník MeSH
Multiple cell membrane alterations have been described in humans and animals with various genetic forms of hypertension and/or dyslipidemia. The aim of our study was to characterize some properties of platelets and/or erythrocytes (cytosolic calcium handling, intracellular pH regulation and thrombin responsiveness) in a new model of genetic hypertension associated with hyperlipidemia-Prague hereditary hypertriglyceridemic (HTG) rats. There were no differences in basal cytosolic Ca2+ values in platelets or erythrocytes of HTG rats and control Wistar rats. Ca2+ influx into erythrocytes was also similar in HTG and control rats. In both strains Ca2+ influx correlated positively with plasma triglycerides. The slope of this relationship was less steep in HTG than in Wistar rats. Cytosolic Ca2+ response to thrombin stimulation was smaller in HTG platelets, which were also characterized by a major reduction of thrombin-induced Mn2+ entry through receptor-operated Ca2+ channels. Platelets of HTG rats had the same basal intracellular pHi values and similar buffering capacity as control rats but their pHi response to thrombin stimulation was substantially reduced. It can be concluded that reduced responsiveness to thrombin stimulation is a major alteration found in platelets of hypertensive hereditary hypertriglyceridemic rats.
Citace poskytuje Crossref.org
Research on Experimental Hypertension in Prague (1966-2009)
