Polyclonal activation of human lymphocytes by Bacillus firmus and its constituents
Language English Country United States Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
8768255
DOI
10.1007/bf02818523
Knihovny.cz E-resources
- MeSH
- Lymphocyte Activation * MeSH
- B-Lymphocytes immunology MeSH
- Bacillus immunology MeSH
- Adult MeSH
- Fetal Blood cytology immunology MeSH
- Humans MeSH
- Peptidoglycan pharmacology MeSH
- Antibodies, Bacterial biosynthesis MeSH
- Aging immunology MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Peptidoglycan MeSH
- Antibodies, Bacterial MeSH
Bacillus firmus strongly stimulates Ig synthesis in the cultures of human peripheral blood mononuclear leukocytes. As apparent from the character of Ig formation and blastic transformation, the stimulation has features of a polyclonal activation of B lymphocytes without substantial participation of T lymphocytes. B firmus is a strong B cell polyclonal activator even for human cord blood lymphocytes. The most striking feature is the strong stimulation of IgA synthesis in both adult and cord blood lymphocytes. Several crude fractions were isolated from B. firmus. None of them exhibited any remarkable enhancement of activity but the cytoplasmic fraction P-40 was clearly more potent than the intact bacilli. On the other hand, cell wall peptidoglycan, a well known polyclonal activator of B cells, had a much lower activity than whole bacteria. The effect of B. firmus on the stimulation of Ig formation is thus relatively complex; it is not caused mainly by peptidoglycan but rather by some cytoplasmic constituents of the bacterium.
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