Ultrastructural nonisotopic mapping of nucleolar transcription sites in onion protoplasts

. 1996 Mar-Apr ; 116 (2) : 253-63.

Jazyk angličtina Země Spojené státy americké Médium print

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/pmid08812981
Odkazy

PubMed 8812981
DOI 10.1006/jsbi.1996.0040
PII: S1047-8477(96)90040-4
Knihovny.cz E-zdroje

The post- and preembedding ultrastructural localization of transcribing rRNA genes has been carried out in nucleoli of permeabilized onion growing root tip protoplasts by means of the nonisotopic bromouridine method. By means of both post- and preembedding approaches, major synthetic sites were identified with morphologically distinct subdomains of dense fibrillar components, with some signal also being associated with nucleolar fibrillar centers and vacuoles. Moreover, labeled medusoid fibrils within distinct domains seen in Lowicryl thin sections likely represent the morphological correlate of transcribing nucleolar genes.

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