Ultrastructural nonisotopic mapping of nucleolar transcription sites in onion protoplasts
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
8812981
DOI
10.1006/jsbi.1996.0040
PII: S1047-8477(96)90040-4
Knihovny.cz E-resources
- MeSH
- Allium ultrastructure MeSH
- Staining and Labeling methods MeSH
- Bromouracil analogs & derivatives MeSH
- Cell Nucleolus ultrastructure MeSH
- Microscopy, Fluorescence methods MeSH
- Transcription, Genetic * MeSH
- Microscopy, Immunoelectron methods MeSH
- Plant Roots ultrastructure MeSH
- Protoplasts ultrastructure MeSH
- RNA, Ribosomal biosynthesis MeSH
- RNA, Plant biosynthesis MeSH
- Uridine analogs & derivatives MeSH
- Vacuoles ultrastructure MeSH
- Tissue Embedding MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 5-bromouridine MeSH Browser
- Bromouracil MeSH
- RNA, Ribosomal MeSH
- RNA, Plant MeSH
- Uridine MeSH
The post- and preembedding ultrastructural localization of transcribing rRNA genes has been carried out in nucleoli of permeabilized onion growing root tip protoplasts by means of the nonisotopic bromouridine method. By means of both post- and preembedding approaches, major synthetic sites were identified with morphologically distinct subdomains of dense fibrillar components, with some signal also being associated with nucleolar fibrillar centers and vacuoles. Moreover, labeled medusoid fibrils within distinct domains seen in Lowicryl thin sections likely represent the morphological correlate of transcribing nucleolar genes.
References provided by Crossref.org
Fluctuations of pol I and fibrillarin contents of the nucleoli
Reproduction of the FC/DFC units in nucleoli
Nucleolar DNA: the host and the guests
