Immunosuppressive effect of bovine seminal ribonuclease on a model of corneal transplantation in rabbit
Jazyk angličtina Země Německo Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
8880158
DOI
10.1007/bf00448804
Knihovny.cz E-zdroje
- MeSH
- aktivace lymfocytů účinky léků MeSH
- aplikace lokální MeSH
- buněčné dělení MeSH
- endoribonukleasy farmakokinetika farmakologie MeSH
- imunoblotting MeSH
- imunosupresivní léčba metody MeSH
- injekce MeSH
- konjunktiva MeSH
- králíci MeSH
- kultivované buňky MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- přední komora oční metabolismus MeSH
- přežívání štěpu účinky léků MeSH
- rejekce štěpu imunologie metabolismus prevence a kontrola MeSH
- skot MeSH
- T-lymfocyty účinky léků imunologie MeSH
- transplantace rohovky * imunologie patologie MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- mužské pohlaví MeSH
- myši MeSH
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- endoribonukleasy MeSH
- ribonuclease SPL MeSH Prohlížeč
BACKGROUND: Bovine seminal ribonuclease (BS RNase) was determined to have a specific suppressive effect on the proliferation of T lymphocytes in vitro. Its immunosuppressive effect was proven in skin grafting in mice as well. METHODS: The immunosuppressive effect of BS RNase was evaluated in tissue cultures and on a model of corneal transplantation in rabbits. The penetration of BS RNase into the anterior chamber was detected by immunoblotting of anterior chamber fluid obtained from animals treated either topically or subconjunctivally. RESULTS: In vitro blastic transformation of mouse T lymphocytes was significantly inhibited by BS RNase (concentrations 15-250 micrograms/ml). No such effect was observed on B lymphocytes. In the rabbit model of corneal graft rejection, BS RNase injected subconjunctivally prolonged mean graft survival time significantly (33.4 days) compared with placebo (salt solution; MST 17.7 days). No BS RNase was detected by immunoblotting in anterior chamber fluid after either topical or subconjunctival application. CONCLUSION: BS RNase showed significant immunosuppressive effect both in the blastic transformation test and in the rabbit high-risk model of corneal transplantation. Negative results of anterior chamber fluid immunoblotting indicate poor absorption of the drug.
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