Aspermatogenic effect of the bull seminal ribonuclease (BS RNase) in the presence of anti-BS RNase antibodies in mice
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články
- MeSH
- epididymis účinky léků patologie MeSH
- fertilita účinky léků MeSH
- imunokomplex farmakologie MeSH
- myši inbrední ICR MeSH
- myši MeSH
- protilátky farmakologie MeSH
- ribonukleasy antagonisté a inhibitory imunologie farmakologie MeSH
- semenné váčky enzymologie MeSH
- skot MeSH
- spermatogeneze účinky léků MeSH
- těhotenství MeSH
- testis účinky léků patologie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- skot MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- imunokomplex MeSH
- protilátky MeSH
- ribonukleasy MeSH
Injection of mouse scrotum with the bull seminal ribonuclease (BS RNase) isolated from bull seminal vesicle fluid inhibited spermatogenesis and caused a decrease in the weight of the testes. Long-term injection of BS RNase evoked the production of antibodies which reached the titre 524,448. These antibodies did not prevent the aspermatogenic action of BS RNase in vivo when a twofold higher amount of this enzyme was injected into mouse scrotum. Aspermatogenesis was reversible in both the first and second part of the experiment. During the period of aspermatogenesis the males were sterile. Increasing the amount of BS RNase injections in the second part of experiments caused aspermatogenesis around 3 months. No malformations were observed among offspring of males recovered from the first stage of aspermatogenesis. The antigen-antibody complex prepared in vitro and injected into testes of mice evoked the same degree of aspermatogenesis as the enzyme itself.
Citace poskytuje Crossref.org
Ribonucleases endowed with specific toxicity for spermatogenic layers
