Leucine metabolism in rats with cirrhosis
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
8907575
DOI
10.1016/s0168-8278(96)80031-6
PII: S0168-8278(96)80031-6
Knihovny.cz E-resources
- MeSH
- Liver Cirrhosis, Experimental chemically induced metabolism MeSH
- Evaluation Studies as Topic MeSH
- Isoleucine metabolism MeSH
- Liver metabolism MeSH
- Keto Acids metabolism MeSH
- Rats MeSH
- Leucine metabolism MeSH
- Carbon Tetrachloride Poisoning metabolism MeSH
- Oxidation-Reduction MeSH
- Perfusion MeSH
- Rats, Wistar MeSH
- Protein Biosynthesis MeSH
- In Vitro Techniques MeSH
- Valine metabolism MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- alpha-ketoisocaproic acid MeSH Browser
- Isoleucine MeSH
- Keto Acids MeSH
- Leucine MeSH
- Valine MeSH
BACKGROUND/AIMS: This study aimed to investigate the pathogenesis of reduced plasma levels of branched-chain amino acids leucine, isoleucine and valine in cirrhosis. METHODS: Cirrhosis was induced by intragastric administration of 36 doses of carbon tetrachloride in olive oil over a period of 12 weeks. Rats treated with oil alone served as controls. The rates of leucine turnover, clearance, oxidation and incorporation into proteins were evaluated using [1-14C]leucine, [4,5-3H]leucine and alpha-keto[1-14C]isocaproate 3 days after the last intragastric treatment in vivo and in the isolated perfused liver. RESULTS: In animals with cirrhosis we observed a profound fall in plasma branched-chain amino acid levels and significant decreases in leucine turnover, oxidation and incorporation into tissue proteins. A more pronounced fall in leucine incorporation in proteins resulted in a significant increase in the oxidized leucine fraction in rats with cirrhosis as compared to controls. Leucine clearance was higher in the cirrhosis group. Concomitant to the fall of whole body leucine turnover, decreases of leucine incorporation into protein and of ketoisocaproic acid decarboxylation were observed in the isolated perfused liver of rats with cirrhosis. However, leucine oxidation was increased compared with control rats. CONCLUSIONS: Our results indicate that the predominant mechanism of the decrease in plasma leucine levels in rats with cirrhosis is an increase in the oxidized leucine fraction associated with a decrease in leucine turnover. An increase in leucine oxidation in the cirrhotic liver is one of the mechanisms involved.
References provided by Crossref.org
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