The in vivo antibody response against exogenous antigens is not influenced by the mouse Bcg (Nramp1) gene
Jazyk angličtina Země Velká Británie, Anglie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
9176118
PubMed Central
PMC1456678
DOI
10.1046/j.1365-2567.1997.00172.x
Knihovny.cz E-zdroje
- MeSH
- buněčné dělení imunologie MeSH
- buňky produkující protilátky imunologie MeSH
- epitopy imunologie MeSH
- erytrocyty imunologie MeSH
- glykosylace MeSH
- inbrední kmeny myší MeSH
- inzulin imunologie MeSH
- lymfatické uzliny imunologie MeSH
- makrofágy imunologie MeSH
- membránové proteiny genetika MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- prezentace antigenu MeSH
- proteiny přenášející kationty * MeSH
- T-lymfocyty imunologie MeSH
- transportní proteiny genetika MeSH
- tvorba protilátek genetika MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- epitopy MeSH
- inzulin MeSH
- membránové proteiny MeSH
- natural resistance-associated macrophage protein 1 MeSH Prohlížeč
- proteiny přenášející kationty * MeSH
- transportní proteiny MeSH
The mouse Nramp1 (Bcg) gene on chromosome 1 exerts pleiotropic effects on macrophage function. The gene is known to affect presentation of mycobacteria, and other antigens in vitro, so that macrophages carrying the resistant Bcg allele better support the proliferation of antigen-specific T cells compared with macrophages of the sensitive phenotype. To determine whether the Bcg allele could affect in vivo the antibody response to antigens not related to mycobacterial infections, we tested the primary and secondary responses to sheep red blood cells (SRBC) and glycosylated bovine insulin (G-insulin) in two pairs of Bcg congenic strains: BALB/c (Bcgs) versus BALB/c.CD2 (Bcgr), and B10.A (Bcgs) versus B10Ar (Bcgr), and in C57BL/10ScSn (B10; Bcgs) and A/J (Bcgr) mice. Furthermore, the antigen-specific proliferative responses of T cells primed in vivo by protein antigens were also tested in Bcg congenic mice. We found no significant difference in in vivo antibody response either to SRBC or G-insulin between the Bcgr and Bcgs strains. The magnitude of in vitro antigen-specific proliferation of lymph node cells sensitized in vivo by hen egg lysozyme (HEL) or chicken ovalbumin (OVA) was also similar in Bcgs and Bcgr congenic mice. However, we have documented a higher antigen-presenting capacity of Bcgr macrophages in in vitro antigen-specific proliferation to OVA. Since the macrophages are the only cells in which the Nramp1 gene is expressed, we suggest that the activity of other types of antigen-presenting cells masks the effect of the Bcgr allele on antigen-presentation in vivo.
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