Inactive fatty acids are unable to flip-flop across the lipid bilayer
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.
Grant support
GM31086
NIGMS NIH HHS - United States
PubMed
9187359
DOI
10.1016/s0014-5793(97)00334-7
PII: S0014-5793(97)00334-7
Knihovny.cz E-resources
- MeSH
- Quinolinium Compounds MeSH
- Fluorescent Dyes MeSH
- Ionophores pharmacology MeSH
- Kinetics MeSH
- Hydrogen-Ion Concentration MeSH
- Lipid Bilayers metabolism MeSH
- Liposomes metabolism MeSH
- Fatty Acids chemistry metabolism MeSH
- Fatty Acid-Binding Proteins MeSH
- Protons MeSH
- Recombinant Proteins * MeSH
- Carrier Proteins metabolism MeSH
- Valinomycin pharmacology MeSH
- Protein Binding MeSH
- Chromatography, High Pressure Liquid MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Names of Substances
- 6-methoxy-N-(3-sulfopropyl)quinolinium MeSH Browser
- acrylodated intestinal fatty acid binding protein, recombinant MeSH Browser
- Quinolinium Compounds MeSH
- Fluorescent Dyes MeSH
- Ionophores MeSH
- Lipid Bilayers MeSH
- Liposomes MeSH
- Fatty Acids MeSH
- Fatty Acid-Binding Proteins MeSH
- Protons MeSH
- Recombinant Proteins * MeSH
- Carrier Proteins MeSH
- Valinomycin MeSH
Free fatty acids (FA) were found which did not acidify liposome interior. This is interpreted as their inability to rapidly flip-flop across the lipid bilayer. However, they were able to partition in lipids as detected directly using HPLC or from the shift of their equilibrium binding to acrylodated intestinal binding protein (ADIFAB) in the presence of vesicles. Various bipolar FA, such as 12-hydroxylauric acid, dicarboxylic acids, or FA with benzene ring at the tail were found to be inactive in this way. A phenomenon of shielding, where an additional alkyl chain or non-polar group can restore the flip-flop activity, is described.
References provided by Crossref.org
Molecular Dynamics Simulations of Mitochondrial Uncoupling Protein 2
Mitochondrial Uncoupling Proteins: Subtle Regulators of Cellular Redox Signaling
Control mechanisms in mitochondrial oxidative phosphorylation
Channel character of uncoupling protein-mediated transport
