Inactive fatty acids are unable to flip-flop across the lipid bilayer
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.
Grantová podpora
GM31086
NIGMS NIH HHS - United States
PubMed
9187359
DOI
10.1016/s0014-5793(97)00334-7
PII: S0014-5793(97)00334-7
Knihovny.cz E-zdroje
- MeSH
- chinolinové sloučeniny MeSH
- fluorescenční barviva MeSH
- ionofory farmakologie MeSH
- kinetika MeSH
- koncentrace vodíkových iontů MeSH
- lipidové dvojvrstvy metabolismus MeSH
- liposomy metabolismus MeSH
- mastné kyseliny chemie metabolismus MeSH
- proteiny vázající mastné kyseliny MeSH
- protony MeSH
- rekombinantní proteiny * MeSH
- transportní proteiny metabolismus MeSH
- valinomycin farmakologie MeSH
- vazba proteinů MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Názvy látek
- 6-methoxy-N-(3-sulfopropyl)quinolinium MeSH Prohlížeč
- acrylodated intestinal fatty acid binding protein, recombinant MeSH Prohlížeč
- chinolinové sloučeniny MeSH
- fluorescenční barviva MeSH
- ionofory MeSH
- lipidové dvojvrstvy MeSH
- liposomy MeSH
- mastné kyseliny MeSH
- proteiny vázající mastné kyseliny MeSH
- protony MeSH
- rekombinantní proteiny * MeSH
- transportní proteiny MeSH
- valinomycin MeSH
Free fatty acids (FA) were found which did not acidify liposome interior. This is interpreted as their inability to rapidly flip-flop across the lipid bilayer. However, they were able to partition in lipids as detected directly using HPLC or from the shift of their equilibrium binding to acrylodated intestinal binding protein (ADIFAB) in the presence of vesicles. Various bipolar FA, such as 12-hydroxylauric acid, dicarboxylic acids, or FA with benzene ring at the tail were found to be inactive in this way. A phenomenon of shielding, where an additional alkyl chain or non-polar group can restore the flip-flop activity, is described.
Citace poskytuje Crossref.org
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