Effect of chemical structure of hydrogels on the adhesion and phenotypic characteristics of human monocytes such as expression of galectins and other carbohydrate-binding sites
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
9212197
DOI
10.1016/s0142-9612(97)00037-9
PII: S0142961297000379
Knihovny.cz E-resources
- MeSH
- Acetylgalactosamine metabolism MeSH
- Biocompatible Materials chemistry metabolism MeSH
- Cell Adhesion physiology MeSH
- Phenotype MeSH
- Gels MeSH
- Heparin metabolism MeSH
- Immunohistochemistry MeSH
- Lectins biosynthesis genetics MeSH
- Humans MeSH
- Mannosides metabolism MeSH
- Methacrylates chemistry metabolism MeSH
- Monocytes cytology metabolism MeSH
- Polymers MeSH
- Surface Properties MeSH
- Prostheses and Implants MeSH
- Gene Expression Regulation genetics MeSH
- Glass MeSH
- Structure-Activity Relationship MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 2-(dimethylamino)ethyl methacrylate MeSH Browser
- Acetylgalactosamine MeSH
- Biocompatible Materials MeSH
- Gels MeSH
- Heparin MeSH
- hydroxyethyl methacrylate MeSH Browser
- Lectins MeSH
- Mannosides MeSH
- methacrylic acid MeSH Browser
- Methacrylates MeSH
- Polymers MeSH
The reactivity of diverse immune aspects to the presence of synthetic polymers represents one of the most important aspects of implantable device biocompatibility. In this report, we show the effect of the chemical structure of a synthetic polymer support on monocyte adhesion and selected phenotypic characteristics in vitro as a model for the initial steps of non-self-recognition of an implant. The extent of monocyte adhesion was significantly influenced by the support chemistry. The highest level of monocyte adhesion was observed on a surface copolymer of 2-hydroxyethyl methacrylate with dimethyl aminoethyl methacrylate relative to results of experiments in which poly(2-hydroxyethyl methacrylate) or the copolymer of 2-hydroxyethyl methacrylate with the sodium salt of methacrylic acid was used. Cell adhesion to the polymers tested and to glass was accompanied by enhanced expression of the carbohydrate-binding sites tested for asialoglycoprotein beta-galactosides such as galectins, beta-N-acetylgalactosamine, alpha-mannoside, specific lectin for heparin as well as the lymphokine-macrophage migration inhibitory factor in the monocytes tested. These results suggest the importance of monocyte adhesion to the biomaterial surface for their development into macrophages and further non-self-recognition of the implanted device.
References provided by Crossref.org
Biological properties of copolymer of 2-hydroxyethyl methacrylate with sulfopropyl methacrylate
