Fed-batch cultivation of bakers' yeast: effect of nutrient depletion and heat stress on cell composition
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
Odkazy
PubMed
9246763
DOI
10.1007/bf02818981
Knihovny.cz E-zdroje
- MeSH
- biomasa MeSH
- dusík nedostatek MeSH
- fosfáty nedostatek MeSH
- fungální proteiny analýza MeSH
- Saccharomyces cerevisiae fyziologie MeSH
- vysoká teplota MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- dusík MeSH
- fosfáty MeSH
- fungální proteiny MeSH
The physiology of a commercial strain of bakers' yeast was studied in terms of the cell composition under different growth conditions and of its response to stress. The study comprised fed-batch experiments since this is the system used in bakers' yeast industry. The classical fed-batch fermentation procedure was modified in that the yeast cells were continuously grown to a steady-state at a dilution rate of 0.1/h in order to achieve more or less the same initial starting point in terms of cell composition. This steady-state culture was then switched to fed-batch concomitantly with exposure to stress. The highest amount of trehalose accumulation was achieved when nutrient depletion and heat stress were applied concomitantly. The highest amount of trehalose, 12%, was attained in cells stressed by both nitrogen depletion and heat stress. The protein content remained constant, although with some oscillations, at a value of 30% throughout this dual stress experiment.
