The fungal elicitor cryptogein is a sterol carrier protein
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
9369212
DOI
10.1016/s0014-5793(97)01193-9
PII: S0014-5793(97)01193-9
Knihovny.cz E-resources
- MeSH
- Algal Proteins * MeSH
- Cholesterol metabolism MeSH
- Ergosterol analogs & derivatives metabolism MeSH
- Spectrometry, Fluorescence MeSH
- Fungal Proteins chemistry metabolism MeSH
- Kinetics MeSH
- Liposomes MeSH
- Phytophthora metabolism MeSH
- Carrier Proteins metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Algal Proteins * MeSH
- Cholesterol MeSH
- cryptogein protein, Phytophthora cryptogea MeSH Browser
- dehydroergosterol MeSH Browser
- Ergosterol MeSH
- Fungal Proteins MeSH
- Liposomes MeSH
- Carrier Proteins MeSH
Cryptogein is a protein secreted by the phytopathogenic pseudo-fungus, Phytophthora cryptogea. It is a basic 10 kDa hydrophilic protein having a hydrophobic pocket and three disulfide bridges. These common features with sterol carrier proteins led us to investigate its possible sterol transfer activity using the fluorescent sterol, dehydroergosterol. The results show that cryptogein has one binding site with strong affinity for dehydroergosterol. Moreover, this protein catalyzes the transfer of sterols between phospholipidic artificial membranes. This is the first evidence for the existence of an extracellular sterol carrier protein and for a molecular activity of cryptogein. This property should contribute to an understanding of the role of cryptogein in plant-microorganism interactions.
References provided by Crossref.org
Plasma membrane order and fluidity are diversely triggered by elicitors of plant defence
