Polyketide synthase gene pksM from Aspergillus terreus expressed during growth phase
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
9438344
DOI
10.1007/bf02826548
Knihovny.cz E-resources
- MeSH
- 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase genetics MeSH
- Acyltransferases genetics MeSH
- Alcohol Oxidoreductases genetics MeSH
- Aspergillus genetics growth & development metabolism MeSH
- 3-Oxoacyl-(Acyl-Carrier-Protein) Reductase MeSH
- Hydro-Lyases genetics MeSH
- DNA, Fungal analysis genetics MeSH
- DNA Probes MeSH
- Gene Expression MeSH
- RNA, Fungal isolation & purification MeSH
- Transcription, Genetic MeSH
- Gene Library MeSH
- Genes, Fungal MeSH
- Nucleic Acid Hybridization MeSH
- Introns MeSH
- Cloning, Molecular MeSH
- Codon, Initiator MeSH
- Ligases genetics MeSH
- Molecular Sequence Data MeSH
- Multienzyme Complexes genetics metabolism MeSH
- Blotting, Northern MeSH
- Open Reading Frames MeSH
- Oxidoreductases genetics MeSH
- Penicillium genetics MeSH
- Plasmids MeSH
- Polymerase Chain Reaction MeSH
- Acyl Carrier Protein genetics MeSH
- Restriction Mapping MeSH
- Amino Acid Sequence MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Sequence Homology, Amino Acid MeSH
- Sequence Alignment MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase MeSH
- 6-methylsalicylic acid synthetase MeSH Browser
- Acyltransferases MeSH
- Alcohol Oxidoreductases MeSH
- 3-Oxoacyl-(Acyl-Carrier-Protein) Reductase MeSH
- Hydro-Lyases MeSH
- DNA, Fungal MeSH
- DNA Probes MeSH
- RNA, Fungal MeSH
- Codon, Initiator MeSH
- Ligases MeSH
- Multienzyme Complexes MeSH
- Oxidoreductases MeSH
- Acyl Carrier Protein MeSH
The polyketide synthase gene pksM was detected in the genomic DNA library of Aspergillus terreus by hybridization with the 6-methylsalicylic acid synthase (6-MSAS) gene of Penicillium patulum as a probe. 9524 bp of the cloned DNA were sequenced and a 5.5 kb open reading frame was revealed. A single intron (62 bp) was identified in the conserved position. Two transcription start points were determined within the 5'-flanking region at 50 and 72 (major) bp upstream from the putative translation initiation codon ATG. The conserved active site motifs for ketosynthase, acyltransferase, dehydratase, ketoreductase and acyl carrier protein were found within the predicted polypeptide consisting of 1803 amino acids. Unlike the P. patulum 6-MSAS gene, the transcription of pksM from A. terreus was observed in the middle of the vegetative growth phase.
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