Polyketide synthase gene pksM from Aspergillus terreus expressed during growth phase

. 1997 ; 42 (5) : 419-30.

Jazyk angličtina Země Spojené státy americké Médium print

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/pmid09438344

The polyketide synthase gene pksM was detected in the genomic DNA library of Aspergillus terreus by hybridization with the 6-methylsalicylic acid synthase (6-MSAS) gene of Penicillium patulum as a probe. 9524 bp of the cloned DNA were sequenced and a 5.5 kb open reading frame was revealed. A single intron (62 bp) was identified in the conserved position. Two transcription start points were determined within the 5'-flanking region at 50 and 72 (major) bp upstream from the putative translation initiation codon ATG. The conserved active site motifs for ketosynthase, acyltransferase, dehydratase, ketoreductase and acyl carrier protein were found within the predicted polypeptide consisting of 1803 amino acids. Unlike the P. patulum 6-MSAS gene, the transcription of pksM from A. terreus was observed in the middle of the vegetative growth phase.

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Mol Gen Genet. 1995 Aug 21;248(3):270-7 PubMed

Gene. 1992 Feb 1;111(1):51-60 PubMed

Ciba Found Symp. 1992;171:88-106; discussion 106-12 PubMed

Cell. 1982 Mar;28(3):563-73 PubMed

Am J Cardiol. 1988 Nov 11;62(15):10J-15J PubMed

Mol Gen Genet. 1995 Nov 15;249(2):162-7 PubMed

J Bacteriol. 1985 May;162(2):704-7 PubMed

Eur J Biochem. 1990 Sep 11;192(2):487-98 PubMed

J Bacteriol. 1995 Nov;177(21):6246-54 PubMed

Annu Rev Genet. 1990;24:37-66 PubMed

Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PubMed

Mol Gen Genet. 1996 Nov 27;253(1-2):1-10 PubMed

Mol Gen Genet. 1992 Nov;235(2-3):205-12 PubMed

Arch Microbiol. 1992;158(1):29-34 PubMed

Gene. 1988 Oct 30;70(2):283-93 PubMed

Science. 1991 May 3;252(5006):675-9 PubMed

Anal Biochem. 1981 Apr;112(2):295-8 PubMed

Plant Cell. 1996 Nov;8(11):2139-50 PubMed

Anal Biochem. 1987 Apr;162(1):156-9 PubMed

Eur J Biochem. 1992 Feb 15;204(1):39-49 PubMed

J Bacteriol. 1995 Aug;177(15):4544-8 PubMed

Can J Microbiol. 1991 Jan;37(1):86-95 PubMed

J Bacteriol. 1995 Aug;177(16):4792-800 PubMed

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