Use of the 6-methylsalicylic-acid-synthase gene as a discriminating marker between Aspergillus terreus and Aspergillus flavipes
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
10997132
DOI
10.1007/bf02816250
Knihovny.cz E-zdroje
- MeSH
- acyltransferasy genetika metabolismus MeSH
- Aspergillus klasifikace enzymologie genetika MeSH
- aspergilóza mikrobiologie MeSH
- DNA fungální genetika MeSH
- fylogeneze MeSH
- geny hub * MeSH
- lidé MeSH
- ligasy genetika metabolismus MeSH
- multienzymové komplexy genetika metabolismus MeSH
- oxidoreduktasy genetika metabolismus MeSH
- polymorfismus genetický MeSH
- restrikční mapování MeSH
- ribozomální DNA genetika MeSH
- RNA ribozomální 5.8S genetika MeSH
- sekvenční analýza DNA MeSH
- technika náhodné amplifikace polymorfní DNA MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 6-methylsalicylic acid synthetase MeSH Prohlížeč
- acyltransferasy MeSH
- DNA fungální MeSH
- ligasy MeSH
- multienzymové komplexy MeSH
- oxidoreduktasy MeSH
- ribozomální DNA MeSH
- RNA ribozomální 5.8S MeSH
In nineteen pathogenic and saprophytic isolates denoted as Aspergillus terreus the presence and restriction pattern of the gene pksM (6-methylsalicylic acid synthase) was determined. Five patterns (A-E) were found and in three isolates the gene was missing. The RAPD (Random Amplified Polymorphic DNA) patterns with three primers were analyzed. The strains with pksM pattern possibly derived from the most common pattern A by single mutation (patterns B-D) were also related by RAPD. Among them, three clades were found. The first one contained saprophytes from Asia, the other two clades contained both European and American pathogens and each of them one saprophyte. The sequences of rDNA region containing 5.8S rDNA and spacers ITS1 and ITS2 were established for representatives of each group and the strains missing the pksM gene. The isolates possessing pksM (although with different restriction patterns) grouped as A. terreus group, whereas the isolates lacking this gene were close to Fennellia flavipes.
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