Sulfate-reducing bacteria (SRB) are often isolated from animals and people with ulcerative colitis and can be involved in the IBD development in the gut-intestine axis. The background of the research consisted of obtaining mixed cultures of SRB communities from healthy mice and mice with colitis, finding variation in the distribution of their morphology, to determine pH and temperature range tolerance and their possible production of hydrogen sulfide in the small-large intestinal environment. The methods: Microscopic techniques, biochemical, microbiological, and biophysical methods, and statistical processing of the results were used. The results: Variation in the distribution of sulfate-reducing microbial communities were detected. Mixed cultures from mice with ulcerative colitis had 1.39 times higher production of H2S in comparison with samples from healthy mice. The species of Desulfovibrio genus play an important role in diseases of the small-large intestine axis. Meta-analysis was also used for the observation about an SRB occurrence in healthy and not healthy individuals and the same as their metabolic processes. Conclusions: This finding is important for its possible correlation with inflammation of the intestine, where the present of SRB in high concentration plays a major part. It can be a good possible indicator of the occurrence of IBD.

Department of Biomedical Sciences for Health University of Milan 20122 Milan Italy

Department of Experimental Biology Faculty of Science Masaryk University Kamenice 753 5 62500 Brno Czech Republic

Department of Plant Origin Foodstuffs Hygiene and Technology Faculty of Veterinary Hygiene and Ecology University of Veterinary and Pharmaceutical Sciences 61242 Brno Czech Republic

Regional Centre of Advanced Technologies and Materials Faculty of Science Palacky University in Olomouc 78371 Olomouc Czech Republic

The Department of Environmental Protection Engineering Faculty of Technology Tomas Bata University in Zlín 76001 Zlín Czech Republic

Zobrazit více v PubMed

Gibson G.R., Cummings J.H., Macfarlane G.T. Growth and activities of sulphate-reducing bacteria in gut contents of health subjects and patients with ulcerative colitis. FEMS Microbiol. Ecol. 1991;86:103–112. doi: 10.1111/j.1574-6968.1991.tb04799.x. DOI

Gibson G.R., Macfarlane S., Macfarlane G.T. Metabolic interactions involving sulphate-reducing and methanogenic bacteria in the human large intestine. FEMS Microbiol. Ecol. 1993;12:117–125. doi: 10.1111/j.1574-6941.1993.tb00023.x. DOI

Cummings J.H., Macfarlane G.T., Macfarlane S. Intestinal Bacteria and Ulcerative Colitis. Curr. Issues Intest. Microbiol. 2003;4:9–20. PubMed

Barton L.L., Hamilton W.A. Sulphate-Reducing Bacteria Environmental and Engineered Systems. Cambridge University Press; Cambridge, UK: 2017.

Loubinoux J., Bronowicji J.P., Pereira I.A. Sulphate-reducing bacteria in human feces and their association with inflammatory diseases. FEMS Microbiol. Ecol. 2002;40:107–112. doi: 10.1111/j.1574-6941.2002.tb00942.x. PubMed DOI

Kováč J., Vítězová M., Kushkevych I. Metabolic activity of sulfate-reducing bacteria from rodents with colitis. Open Med. 2018;13:344–349. doi: 10.1515/med-2018-0052. PubMed DOI PMC

Kushkevych I., Vítězová M., Fedrová P., Vochyanová Z., Paráková L., Hošek J. Kinetic properties of growth of intestinal sulphate-reducing bacteria isolated from healthy mice and mice with ulcerative colitis. Acta Vet. Brno. 2017;86:405–411. doi: 10.2754/avb201786040405. DOI

Kushkevych I., Fafula R., Parak T., Bartoš M. Activity of Na+/K+-activated Mg2+-dependent ATP hydrolase in the cell-free extracts of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9. Acta Vet. Brno. 2015;84:3–12. doi: 10.2754/avb201585010003. DOI

Kushkevych I.V. Activity and kinetic properties of phosphotransacetylase from intestinal sulfate-reducing bacteria. Acta Biochem. Pol. 2015;62:1037–1108. doi: 10.18388/abp.2014_845. PubMed DOI

Kushkevych I.V. Kinetic Properties of Pyruvate Ferredoxin Oxidoreductase of Intestinal Sulfate-Reducing Bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9. Pol. J. Microbiol. 2015;64:107–114. PubMed

Loubinoux J., Mory F., Pereira I.A., Le Faou A.E. Bacteremia caused by a strain of Desulfovibrio related to the provisionally named Desulfovibrio fairfieldensis. J. Clin. Microbiol. 2000;38:931–934. PubMed PMC

Pitcher M.C., Cummings J.H. Hydrogen sulphide: A bacterial toxin in ulcerative colitis? Gut. 1996;39:1–4. doi: 10.1136/gut.39.1.1. PubMed DOI PMC

Florin T.H., Neale G., Goretski S. Sulfate in food and beverages. J. Food Compos. Anal. 1993;6:140–151. doi: 10.1006/jfca.1993.1016. DOI

Kushkevych I., Dordević D., Vítězová M., Kollár P. Cross-correlation analysis of the Desulfovibrio growth parameters of intestinal species isolated from people with colitis. Biologia. 2018;73:1137–1143. doi: 10.2478/s11756-018-0118-2. DOI

Kushkevych I., Dordević D., Vítězová M. Analysis of pH dose-dependent growth of sulfate-reducing bacteria. Open Med. 2019;14:66–74. doi: 10.1515/med-2019-0010. PubMed DOI PMC

Kushkevych I., Dordević D., Kollar P. Analysis of physiological parameters of Desulfovibrio strains from individuals with colitis. Open Life Sci. 2018;13:481–488. doi: 10.1515/biol-2018-0057. PubMed DOI PMC

Kushkevych I., Vítězová M., Kos J., Kollár P., Jampilek J. Effect of selected 8-hydroxyquinoline-2-carboxanilides on viability and sulfate metabolism of Desulfovibrio piger. J. Appl. Biomed. 2018;16:241–246. doi: 10.1016/j.jab.2018.01.004. DOI

Kushkevych I., Dordević D., Vítězová M. Toxicity of hydrogen sulfide toward sulfate-reducing bacteria Desulfovibrio piger Vib-7. Arch. Microbiol. 2019;201:389–397. doi: 10.1007/s00203-019-01625-z. PubMed DOI

Kushkevych I., Kollar P., Suchy P., Parak T., Pauk K., Imramovsky A. Activity of selected salicylamides against intestinal sulfate-reducing bacteria. Neuroendocrinol. Lett. 2015;36:106–113. PubMed

Kushkevych I., Kollar P., Ferreira A.L., Palma D., Duarte A., Lopes M.M., Bartos M., Pauk K., Imramovsky A., Jampilek J. Antimicrobial effect of salicylamide derivatives against intestinal sulfate-reducing bacteria. J. Appl. Biomed. 2016;14:125–130. doi: 10.1016/j.jab.2016.01.005. DOI

Kushkevych I., Kos J., Kollar P., Kralova K., Jampilek J. Activity of ring-substituted 8-hydroxyquinoline-2-carboxanilides against intestinal sulfate-reducing bacteria Desulfovibrio piger. Med. Chem. Res. 2018;27:278–284. doi: 10.1007/s00044-017-2067-7. DOI

Loubinoux J., Valente F.M.A., Pereira I.A.C. Reclassification of the only species of the genus Desulfomonas, Desulfomonas pigra, as Desulfovibrio piger comb. nov. Int. J. Syst. Evol. Microbiol. 2002;52:1305–1308. PubMed

Postgate J.R. The Sulfate Reducing Bacteria. Cambridge University Press; Cambridge, UK: 1984.

Rowan F.E., Docherty N.G., Coffey J.C., O’Connell P.R. Sulphate-reducing bacteria and hydrogen sulphide in the aetiology of ulcerative colitis. Br. J. Surg. 2009;96:151–158. doi: 10.1002/bjs.6454. PubMed DOI

Kushkevych I., Vítězová M., Vítěz T., Bartoš M. Production of biogas: Relationship between methanogenic and sulfate-reducing microorganisms. Open Life Sci. 2017;12:82–91.

Kushkevych I., Vítězová M., Vítěz T., Kovac J., Kaucká P., Jesionek W., Bartoš M., Barton L. A new combination of substrates: Biogas production and diversity of the methanogenic microorganisms. Open Life Sci. 2018;13:119–128. doi: 10.1515/biol-2018-0017. PubMed DOI PMC

Kushkevych I., Kováč J., Vítězová M., Vítěz T., Bartoš M. The diversity of sulfate-reducing bacteria in the seven bioreactors. Arch. Microbiol. 2018;200:945–950. doi: 10.1007/s00203-018-1510-6. PubMed DOI

Kushkevych I., Dordević D., Kollar P., Vítězová M., Drago L. Hydrogen Sulfide as a Toxic Product in the Small–Large Intestine Axis and its Role in IBD Development. J. Clin. Med. 2019;8:1054. doi: 10.3390/jcm8071054. PubMed DOI PMC

Mallett R., Hagen-Zanker J., Slater R., Duvendack M. The benefits and challenges of using systematic reviews in international development research. J. Dev. Eff. 2012;4:445–455. doi: 10.1080/19439342.2012.711342. DOI

Kováč J., Kushkevych I. New modification of cultivation medium for isolation and growth of intestinal sulfate-reducing bacteria; Proceedings of the International PhD Students Conference MendelNet; Brno, Czech Republic. 6–7 November 2019; pp. 702–707.

Stan-Lotter H., Leuko S., Legat A., Fendrihan S. The Assessment of the Viability of Halophilic Microorganisms in Natural Communities. Methods Microbiol. 2006;35:569–584.

Cline J.D. Spectrophotometric determination of hydrogen sulfide in natural water. Limnol. Oceanogr. 1969;14:454–458. doi: 10.4319/lo.1969.14.3.0454. DOI

Bailey N.T.J. Statistical Methods in Biology. Cambridge University Press; Cambridge, UK: 1995.

Coutinho C.M.L.M., Coutinho-Silva R., Zinkevich V., Pearce C.B., Ojcius D.M., Beech I. Sulphate-reducing bacteria from ulcerative colitis patients induce apoptosis of gastrointestinal epithelial cells. Microb. Pathog. 2017;112:126–134. doi: 10.1016/j.micpath.2017.09.054. PubMed DOI

Zinkevich V.V., Beech I.B. Screening of sulfate-reducing bacteria in colonoscopy samples from healthy and colitic human gut mucosa. FEMS Microbiol. Ecol. 2000;34:147–155. doi: 10.1111/j.1574-6941.2000.tb00764.x. PubMed DOI

Attene-Ramos M.S., Wagner E.D., Plewa M.J., Gaskins H.R. Evidence that hydrogen sulfide is a genotoxic agent. Mol. Cancer Res. 2006;4:9–14. doi: 10.1158/1541-7786.MCR-05-0126. PubMed DOI

Beauchamp R.O., Bus J.S., Popp J.A., Boreiko C.J., Andjelkovich D.A., Leber P. A critical review of the literature on hydrogen sulfide toxicity. CRC Crit. Rev. Toxicol. 1984;13:25–97. doi: 10.3109/10408448409029321. PubMed DOI

Blachier F., Davila A.M., Mimoun S. Luminal sulfide and large intestine mucosa: Friend or foe? Amino Acids. 2010;39:335–347. doi: 10.1007/s00726-009-0445-2. PubMed DOI

Grieshaber M.K., Völkel S. Animal adaptations for tolerance and exploitation of poisonous sulfide. Annu. Rev. Physiol. 1998;60:33–53. doi: 10.1146/annurev.physiol.60.1.33. PubMed DOI

Černý M., Vítězová M., Vítěz T., Bartoš M., Kushkevych I. Variation in the Distribution of Hydrogen Producers from the Clostridiales Order in Biogas Reactors Depending on Different Input Substrates. Energies. 2018;11:3270. doi: 10.3390/en11123270. DOI

Wallace J.L., Ferraz J.G., Muscara M.N. Hydrogen sulfide: An endogenous mediator of resolution of inflammation and injury. Antioxid. Redox Signal. 2012;17:58–67. doi: 10.1089/ars.2011.4351. PubMed DOI PMC

Szabó C. Hydrogen sulphide and its therapeutic potential. Nat. Rev. Drug Discov. 2007;6:917–935. doi: 10.1038/nrd2425. PubMed DOI

Levitt M.D., Furne J., Springfield J., Suarez F., DeMaster E. Detoxification of hydrogen sulfide and methanethiol in the cecal mucosa. J. Clin. Investing. 1999;104:1107–1114. doi: 10.1172/JCI7712. PubMed DOI PMC

Mottawea W., Chiang C.K., Mühlbauer M., Starr A.E., Butcher J., Abujamel T., Hajibabaei M. Altered intestinal microbiota–host mitochondria crosstalk in new onset Crohn’s disease. Nat. Commun. 2016;7:13419. doi: 10.1038/ncomms13419. PubMed DOI PMC

The impact of 3-sulfo-taurolithocholic acid on ATPase activity in patients' colorectal cancer and normal colon tissues, and its hepatic effects in rodents

NADH and NADPH peroxidases as antioxidant defense mechanisms in intestinal sulfate-reducing bacteria

Sulfur content in foods and beverages and its role in human and animal metabolism: A scoping review of recent studies

ATPase Activity of the Subcellular Fractions of Colorectal Cancer Samples under the Action of Nicotinic Acid Adenine Dinucleotide Phosphate

Microscopic Methods for Identification of Sulfate-Reducing Bacteria from Various Habitats

Intestinal Microbiota and Perspectives of the Use of Meta-Analysis for Comparison of Ulcerative Colitis Studies

Hydrogen sulfide toxicity in the gut environment: Meta-analysis of sulfate-reducing and lactic acid bacteria in inflammatory processes

Sulfate-Reducing Bacteria of the Oral Cavity and Their Relation with Periodontitis-Recent Advances

Evaluation of Physiological Parameters of Intestinal Sulfate-Reducing Bacteria Isolated from Patients Suffering from IBD and Healthy People

Adenosine-5'-Phosphosulfate- and Sulfite Reductases Activities of Sulfate-Reducing Bacteria from Various Environments

Recent Advances in Metabolic Pathways of Sulfate Reduction in Intestinal Bacteria