Intestinal sulfate-reducing bacteria are often isolated from patients with inflammatory bowel disease, including ulcerative colitis, and can be involved in the development of gut inflammation. A comparison of the metabolism of intestinal sulfate-reducing bacteria isolated from individuals with colitis and healthy controls using statistical analysis has never been studied and described before. The aim of our research was to evaluate the parameters of dissimilatory sulfate reduction in Desulfovibrio species that were isolated from the feces of healthy objects and individuals with colitis. Principal component analysis indicates that the strains that were isolated from individuals with colitis grouped in the same cluster by biomass accumulation and sulfide production, same as the strains isolated from healthy individuals. Sulfate and lactate consumption measured over time showed negative correlation (Pearson correlations, p<0.01), healthy: -0.760; colitis: -0.770; healthy: -0.828; colitis: -0.847, respectively. The calculated linear regression (R2) was lower in biomass accumulation and hydrogen sulfide production, 0.531; 0.625 respectively. Thus, biomass accumulation and sulfide production, together with measured kinetic parameters play an important factor in bowel inflammation, including ulcerative colitis. Additionally, acetate production can also synergize with H2S, while sulfate consumption and lactate oxidation likely represent minor factors in bowel disease.

Department of Experimental Biology Faculty of Science Masaryk University Kamenice 753 5 625 00 Brno Czech Republic

Department of Human Pharmacology and Toxicology Faculty of Pharmacy University of Veterinary and Pharmaceutical Sciences Brno Czech Republic

Department of Plant Origin Foodstuffs Hygiene and Technology Faculty of Veterinary Hygiene and Ecology University of Veterinary and Pharmaceutical Sciences Brno Czech Republic

Zobrazit více v PubMed

Hirano A, Umeno J, Okamoto Y, Shibata H, Ogura Y, Moriyama T. Comparison of the microbial community structure between inflamed and non-inflamed sites in patients with ulcerative colitis. J Gastroenterol Hepatol. 2018 Feb 20. PubMed

Gibson GR, Cummings JH, Macfarlane GT. Growth and activities of sulphate-reducing bacteria in gut contents of health subjects and patients with ulcerative colitis. FEMS Microbiol Ecol. 1991;86:103–112.

Gibson GR, Macfarlane S, Macfarlane GT. Metabolic interactions involving sulphate-reducing and methanogenic bacteria in the human large intestine. FEMS Microbiol Ecol. 1993;12:117–125.

Kushkevych I, Kollar P, Suchy P, Parak K, Pauk K, Imramovsky A. Activity of selected salicylamides against intestinal sulfate-reducing bacteria. Neuroendocrinol Lett. 2015;36:106–113. PubMed

Figliuolo VR, Dos Santos LM, Abalo A, Nanini H, Santos A, Brittes NM. Sulfate-reducing bacteria stimulate gut immune responses and contribute to inflammation in experimental colitis. Life Sci. 2017;189:29–38. PubMed

Håkansson Å, Tormo-Badia N, Baridi A, Xu J, Molin G, Hagslätt ML. Immunological alteration and changes of gut microbiota after dextran sulfate sodium (DSS) administration in mice. Clin Exp Med. 2015;15:107–120. PubMed PMC

Barton LL, Hamilton WA. Sulphate-Reducing Bacteria. Environmental and Engineered Systems. Cambridge University Press; 2010.

Cummings JH, Macfarlane GT, Macfarlane S.. Intestinal Bacteria and Ulcerative Colitis. Curr Issues Intest Microbiol. 2003;4:9–20. PubMed

Loubinoux J, Mory F, Pereira IA, Le Faou AE. Bacteremia caused by a strain of Desulfovibrio related to the provisionally named Desulfovibrio fairfieldensis. J Clin Microbiol. 2000;38:931–934. PubMed PMC

Loubinoux J, Bronowicji JP, Pereira IA. Sulphate-reducing bacteria in human feces and their association with inflammatory diseases. FEMS Microbiol Ecol. 2002;40:107–112. PubMed

Loubinoux J, Valente FMA., Pereira IAC. Reclassification of the only species of the genus Desulfomonas Desulfomonaspigra as Desulfovibrio piger comb. nov. Int J of System and Evol Microbiol. 2002;52:1305–1308. PubMed

Pitcher MC, Cummings JH. Hydrogen sulphide: a bacterial toxin in ulcerative colitis? Gut. 1996;39:1–4. PubMed PMC

Rowan FE, Docherty NG, Coffey JC, O’Connell PR. Sulphate-reducing bacteria and hydrogen sulphide in the aetiology of ulcerative colitis. British J Surgery. 2009;96:151–158. PubMed

Kushkevych I, Kollar P., Ferreira AL, Palma D. Antimicrobial effect of salicylamide derivatives against intestinal sulfate-reducing-bacteria. J Appl Biomed. 2016;14:125–130.

Kushkevych I, Vítězová M, Kos J, Kollár P, Jampílek J. Effect of selected 8-hydroxyquinoline-2-carboxanilides on viability and sulfate metabolism of Desulfovibrio piger. J. App Biomed. 2018;16:1–6.

Attene-Ramos MS, Wagner ED, Plewa MJ, Gaskins HR.. Evidence that hydrogen sulfide is a genotoxic agent. Mol Cancer Res. 2006;4:9–14. PubMed

Kovac J, Kushkevych I. New modification of cultivation medium for isolation and growth of intestinal sulfate-reducing bacteria. ProceedofInternat PhD Stud Conf MendelNet. 2017. p. 702–707.

Kushkevych IV. Kinetic Properties of Pyruvate Ferredoxin Oxidoreductase of Intestinal Sulfate-Reducing Bacteria Desulfovibrio piger Vib-7 and Desulfomicrobiumsp. Rod-9. Polish J Microbiol. 2015;64:107–114. PubMed

Kushkevych I, Fafula R, Parak T, Bartos M. Activity of Na+/ K+-activated Mg2+-dependent ATP hydrolase in the cell-free extracts of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9. Acta Vet Brno. 2015;84:3–12.

Kushkevych IV. Activity and kinetic properties of phosphotransacetylase from intestinal sulfate-reducing bacteria. Acta Biochemica Polonica. 2015;62:1037–108. PubMed

Kushkevych I, Vítězová M, Fedrová M, Vochyanová Z, Paráková L, Hošek J.. Kinetic properties of growth of intestinal sulphate-reducing bacteria isolated from healthy mice and mice with ulcerative colitis. Acta Vet Brno. 2017;86:405–411.

Kushkevych IV. Identification of sulfate-reducing bacteria strains of human large intestine. Stud Biol. 2013;7:115–124.

Postgate JR. The sulfate-reducing bacteria. Cambridge University Press; Cambridge: 1984.

Kolmert A, Wikstrom P, Hallberg KB. A fast and simple turbidimetric method for the determination of sulfate in sulfate-reducing bacterial cultures. J Microbiol Methods. 2000;41:179–184. PubMed

Cline JD. Spectrophotometric determination of hydrogen sulfide in natural water. Limnol and Ocean. 1969;14:454–458.

Bailey NTJ. Statistical Methods in Biology. Cambridge University Press; 1995.

Kushkevych I, Vítězová M, Vítěz T, Bartoš M.. Production of biogas: relationship between methanogenic and sulfate-reducing microorganisms. Open Life Sciences. 2017;12:82–91.

Kushkevych I, Kováč J, Vítězová M, Vítěz T, Bartoš M.. The diversity of sulfate-reducing bacteria in the seven bioreactors. Arch Microbiol. 2018;200(6):945–950. PubMed

Kushkevych I, Dordević D, Vítězová M, Kollár P.. Cross-correlation analysis of the Desulfovibrio growth parameters of intestinal species isolated from people with colitis. Biologia. 2018;73(11):1137–1143.

Kushkevych IV. Dissimilatory sulfate reduction in the intestinal sulfate-reducing bacteria. Studia Biologica. 2016;10:197–228.

Griesbeck C, Schutz M, Schodl T, Bathe S, Nausch L, Mederer N. Mechanism of sulfide-quinone reductase investigated using site-directed mutagenesis and sulfur analysis. Biochemistry. 2002;41:11552–11565. PubMed

Coutinho CMLM., Coutinho-Silva R, Zinkevich V, Pearce CB, Ojcius DM, Beech I. Sulphate-reducing bacteria from ulcerative colitis patients induce apoptosis of gastrointestinal epitheli-alcells. Microbial pathogenesis. 2017;112:126–134. PubMed

Advances in gut microbiota functions in inflammatory bowel disease: Dysbiosis, management, cytotoxicity assessment, and therapeutic perspectives

The impact of 3-sulfo-taurolithocholic acid on ATPase activity in patients' colorectal cancer and normal colon tissues, and its hepatic effects in rodents

Comparison of microbial communities and the profile of sulfate-reducing bacteria in patients with ulcerative colitis and their association with bowel diseases: a pilot study

Sulfur content in foods and beverages and its role in human and animal metabolism: A scoping review of recent studies

ATPase Activity of the Subcellular Fractions of Colorectal Cancer Samples under the Action of Nicotinic Acid Adenine Dinucleotide Phosphate

Microscopic Methods for Identification of Sulfate-Reducing Bacteria from Various Habitats

Intestinal Microbiota and Perspectives of the Use of Meta-Analysis for Comparison of Ulcerative Colitis Studies

Possible synergy effect of hydrogen sulfide and acetate produced by sulfate-reducing bacteria on inflammatory bowel disease development

Hydrogen sulfide toxicity in the gut environment: Meta-analysis of sulfate-reducing and lactic acid bacteria in inflammatory processes

Sulfate-Reducing Bacteria of the Oral Cavity and Their Relation with Periodontitis-Recent Advances

Evaluation of Physiological Parameters of Intestinal Sulfate-Reducing Bacteria Isolated from Patients Suffering from IBD and Healthy People

Adenosine-5'-Phosphosulfate- and Sulfite Reductases Activities of Sulfate-Reducing Bacteria from Various Environments

The Characterization of Enterococcus Genus: Resistance Mechanisms and Inflammatory Bowel Disease

Recent Advances in Metabolic Pathways of Sulfate Reduction in Intestinal Bacteria

ATP sulfurylase activity of sulfate-reducing bacteria from various ecotopes

Occurrence of Thermophilic Microorganisms in Different Full Scale Biogas Plants

Hydrogen Sulfide Effects on the Survival of Lactobacilli with Emphasis on the Development of Inflammatory Bowel Diseases

The Sulfate-Reducing Microbial Communities and Meta-Analysis of Their Occurrence during Diseases of Small-Large Intestine Axis

Hydrogen Sulfide as a Toxic Product in the Small-Large Intestine Axis and its Role in IBD Development