We report a highly sensitive and selective multiplex assay by empowering an electrochemical DNA sensor with isothermal rolling circle amplification. The assay could simultaneously detect and discriminate three common entero-pathogens in a single reaction, with femtomolar sensitivity. It is useful for field- or resource-limited settings.

Biosensors Laboratory Department of Biomedical Engineering Faculty of Engineering Mahidol University Nakhon Pathom Thailand

Carl R Woese Institute for Genomic Biology University of Illinois at Urbana Champaign Urbana IL 61801 USA

Centre of Excellence for Omics Driven Computational Biodiscovery and Department of Biotechnology Faculty of Applied Sciences AIMST University Kedah 08100 Malaysia

College of Food Science and Technology Shanghai Ocean University Shanghai 201306 China

Division of Physical Biology and Bioimaging Center Shanghai Synchrotron Radiation Facility CAS Key Laboratory of Interfacial Physics and Technology Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai China

Institute for Nanomaterials Advanced Technologies and Innovation Technical University of Liberec Studentská 1402 2 461 17 Liberec Czech Republic

Pilot Plant and Development Training Institute Nanoscience and Nanotechnology Research Cluster King Mongkut's University of Technology Thonburi Bang Khun Thian Campus Bangkok 10150 Thailand

School of Bioresources and Technology King Mongkut's University of Technology Thonburi Thailand

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