Use of the 6-methylsalicylic-acid-synthase gene as a discriminating marker between Aspergillus terreus and Aspergillus flavipes
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
10997132
DOI
10.1007/bf02816250
Knihovny.cz E-resources
- MeSH
- Acyltransferases genetics metabolism MeSH
- Aspergillus classification enzymology genetics MeSH
- Aspergillosis microbiology MeSH
- DNA, Fungal genetics MeSH
- Phylogeny MeSH
- Genes, Fungal * MeSH
- Humans MeSH
- Ligases genetics metabolism MeSH
- Multienzyme Complexes genetics metabolism MeSH
- Oxidoreductases genetics metabolism MeSH
- Polymorphism, Genetic MeSH
- Restriction Mapping MeSH
- DNA, Ribosomal genetics MeSH
- RNA, Ribosomal, 5.8S genetics MeSH
- Sequence Analysis, DNA MeSH
- Random Amplified Polymorphic DNA Technique MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 6-methylsalicylic acid synthetase MeSH Browser
- Acyltransferases MeSH
- DNA, Fungal MeSH
- Ligases MeSH
- Multienzyme Complexes MeSH
- Oxidoreductases MeSH
- DNA, Ribosomal MeSH
- RNA, Ribosomal, 5.8S MeSH
In nineteen pathogenic and saprophytic isolates denoted as Aspergillus terreus the presence and restriction pattern of the gene pksM (6-methylsalicylic acid synthase) was determined. Five patterns (A-E) were found and in three isolates the gene was missing. The RAPD (Random Amplified Polymorphic DNA) patterns with three primers were analyzed. The strains with pksM pattern possibly derived from the most common pattern A by single mutation (patterns B-D) were also related by RAPD. Among them, three clades were found. The first one contained saprophytes from Asia, the other two clades contained both European and American pathogens and each of them one saprophyte. The sequences of rDNA region containing 5.8S rDNA and spacers ITS1 and ITS2 were established for representatives of each group and the strains missing the pksM gene. The isolates possessing pksM (although with different restriction patterns) grouped as A. terreus group, whereas the isolates lacking this gene were close to Fennellia flavipes.
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Folia Microbiol (Praha). 1964 Nov;90:383-6 PubMed
Folia Microbiol (Praha). 1997;42(5):419-30 PubMed
J Clin Microbiol. 1993 May;31(5):1117-21 PubMed
Mol Biol Evol. 1987 Jul;4(4):406-25 PubMed
Comput Appl Biosci. 1994 Sep;10(5):569-70 PubMed
Comput Appl Biosci. 1994 Apr;10(2):189-91 PubMed
Mol Biol Evol. 1996 Jul;13(6):809-17 PubMed
Appl Environ Microbiol. 1994 Jul;60(7):2408-14 PubMed
J Clin Microbiol. 1992 Nov;30(11):2991-3 PubMed
Appl Microbiol Biotechnol. 1998 Jul;50(1):98-104 PubMed
Curr Genet. 1991 Nov;20(5):391-6 PubMed
Appl Environ Microbiol. 1995 Jan;61(1):40-3 PubMed
Mol Gen Genet. 1996 Nov 27;253(1-2):1-10 PubMed
Eur J Biochem. 1990 Sep 11;192(2):487-98 PubMed
Theor Appl Genet. 1995 Jan;90(1):27-32 PubMed
