A strain of starch-assimilating yeast, Saccharomycopsis capsularis, isolated from Indian cereal-based fermented foods, produced significant levels of extracellular alpha-amylase and glucoamylase. The enzymes reached their peak activities during the stationary phase at the end of the 5th and 4th day of cultivation, respectively. The amylase yields were maximized by a proper choice of carbon and nitrogen sources, starting pH of the culture medium and growth temperature. High activities of the enzymes were obtained through inexpensive agricultural commodities, such as wheat bran and corn meal as carbon sources, and defatted soybean meal and peanut meal as nitrogen sources. A temperature of 28-32 degrees C and an initial pH of 4.5-5.0 were optimum. The crude amylase mixture could liquefy and saccharify a 1% starch solution completely in 24 h at 50 degrees C.

Department of Microbiology Panjab University Chandigarh India

Zobrazit více v PubMed

World J Microbiol Biotechnol. 1990 Mar;6(1):19-22 PubMed

Biochem Cell Biol. 1987 Oct;65(10):899-908 PubMed

World J Microbiol Biotechnol. 1990 Mar;6(1):15-8 PubMed

Folia Microbiol (Praha). 1978;23(6):423-7 PubMed

Antonie Van Leeuwenhoek. 1980;46(4):399-405 PubMed

Appl Environ Microbiol. 1980 Jan;39(1):41-7 PubMed

Nature. 1950 Sep 9;166(4219):444-5 PubMed

Can J Microbiol. 1986 Jan;32(1):47-51 PubMed

Appl Environ Microbiol. 1982 Aug;44(2):301-7 PubMed

Appl Environ Microbiol. 1985 Dec;50(6):1474-82 PubMed