Conformational properties of DNA strands containing guanine-adenine and thymine-adenine repeats
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
9490799
PubMed Central
PMC147418
DOI
10.1093/nar/26.6.1509
PII: gkb259
Knihovny.cz E-zdroje
- MeSH
- cirkulární dichroismus MeSH
- dinukleotidové repetice * MeSH
- DNA chemie genetika MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- heteroduplexy nukleové kyseliny chemie genetika MeSH
- konformace nukleové kyseliny * MeSH
- sekvence nukleotidů MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA MeSH
- heteroduplexy nukleové kyseliny MeSH
CD spectroscopy and PAGE were used to cooperatively analyze melting conformers of DNA strands containing GA and TA dinucleotide repeats. The 20mer (GA)10 formed a homoduplex in neutral solutions containing physiological concentrations of salts and this homoduplex was not destabilized even in the terminal (GA)3 hexamers of (GA)3(TA)4(GA)3, although the central (TA)4 portion of this oligonucleotide preserved the conformation adopted by (TA)10. This observation demonstrates that homoduplexes of alternating GA and TA sequences can co-exist in a single DNA molecule. Another 20mer, (GATA)5, adopted as a whole either the AT duplex, like (TA)10, or the GA duplex, like (GA)10, and switched between them reversibly. The concentration of salt controlled the conformational switching. Hence, guanine and thymine share significant properties regarding complementarity to adenine, while the TA and GA sequences can stack in at least two mutually compatible ways within the DNA duplexes analyzed here. These properties extend our knowledge of non-canonical structures of DNA.
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